PubMed İndeksli Yayınlar Koleksiyonu
https://hdl.handle.net/20.500.12587/7
PubMed Indexed Publications Collection2024-03-28T22:01:06ZDetecting pain severity with full cup test in painful diabetic peripheral neuropathy
https://hdl.handle.net/20.500.12587/13979
Detecting pain severity with full cup test in painful diabetic peripheral neuropathy
Say, Bahar; Ergün, Ufuk; Yıldız, Ayşe; Alpua, Murat; Arıkan, Şenay Durmaz; Turgal, Ebru
Objectives: The aim of this study was to test the utility and validity of the full cup test (FCT) to assess the severity of pain in subjects with painful diabetic peripheral neuropathy (PDPN). Methods: Subjects with diabetic PDPN were enrolled for this prospective, cross-sectional study. Other causes of PDPN and subjects with cognitive impairment were excluded. The diagnosis of neuropathic pain was made using the results of a physical examination and the Douleur Neuropathique 4 questionnaire. Pain severity was assessed with a FCT and a visual analog scale (VAS) administered before and after treatment. The correlation of FCT with VAS was evaluated to examine validity. Results: A total of 43 (33 female, 10 male) subjects were included. The mean age was 61.9±8.25 years and the mean disease duration was 13.02±7.6 years. Type I diabetes mellitus (DM) was present in 2 (4.7%) subjects and Type II DM in 41 (95.3%) subjects. The mean glycated hemoglobin level was 8.9±1.9 mmol/mol. When the mean VAS and FCT scores were analyzed, the results were 6.7±2.05 and 66.35±23.2, respectively, pretreatment and 4.6±2.2 and 41.36±23.5 posttreatment, which were both statistically significant (p<0.001, p<0.001). The mean control period was 23.4 days (min–max: 15-30 days). The VAS and FCT scores in pretreatment and posttreatment demonstrated a high positive correlation (rs =0.86, p<0.001; rs =0.843, p<0.001). Conclusion: The FCT can be useful to detect pain severity in PDPN.; Amaç: Dolu bardak testinin (DBT) ağrılı diyabetik periferal nöropati (ADPN)’de kullanımını ve geçerliliğini test etmek. Gereç ve Yöntem: Bu kesitsel ve prospektif çalışmaya ADPN olan olgular alındı. Çalışma popülasyonunda periferal nöropati yapacak diğer nedenler ve kognitif yetmezlik dışlandı. Periferik nöropati tanısı muayene ve Douleur Neuropathique 4 (DN4) anketi kullanılarak gösterildi. Ağrı şiddeti tedavi öncesi ve sonrasında DBT ve Vizüel Analog Skala (VAS) ile değerlendirildi. Geçerlilik için DBT’nin VAS ile korelasyonuna bakıldı. Bulgular: Çalışmaya 43 (33 kadın, 10 erkek) olgu alındı. Ortalama yaş 61.9±8.25, ortalama hastalık süresi 13.02±7.6 yıldı. Tip I DM 2 (%4.7) olguda, Tip II DM 41 (%95.3) olguda mevcuttu. HbA1c düzeyi ortalama 8.9±1.9 mmol/mol’du. Ağrı şiddetinin değerlendirilmesinde kullanılan VAS ve DBT tedavi öncesi ve sonrası karşılaştırıldığında ortalama skorlar tedavi öncesinde 6.7±2.05, 66.35±23.2, tedavi sonrasında ise 4.6±2.2, 41.36±23.5 bulundu. Sonuçlar istatistiksel olarak anlamlıydı (p<0.001, p<0.001). Olgularda ortalama kontrol süresi 23.4 (minimum–maksimum: 15–30) gün oldu. Ağrı şiddet ölçeği DBT, tedavi öncesi ve sonrasında VAS ile yüksek korele bulundu (rs =0.86, p<0.001; rs =0.843, p<0.001). Sonuç: Dolu bardak testi ADPN’de ağrı şiddetini belirlemede kullanışlı olabilir.
2020-01-01T00:00:00ZTherapeutic Treatment with Abdominal Adipose Mesenchymal Cells Does Not Prevent Elastase-Induced Emphysema in Rats
https://hdl.handle.net/20.500.12587/13976
Therapeutic Treatment with Abdominal Adipose Mesenchymal Cells Does Not Prevent Elastase-Induced Emphysema in Rats
Gülhan, Pınar Yıldız; Ekici, Mehmet Savaş; Ekici, Aydanur; Niyaz, Mehmet; Gülhan, Muhammet; Ercin, Mustafa Emre; Aksoy, Nurkan
OBJECTIVES: Emphysema and chronic bronchitis have different pathophysiologies but both are significant components of chronic obstructive lung disease (COPD). The levels of Matrix metalloproteinase (MMP)-9 in the bronchoalveloar lavage fluid (BALF) and in serum indicate the presence of emphysema. Intratracheal administration of elastase has been used to create a rat model of emphysema. Adipose tissue-derived mesenchymal stem cells (MSC) have been postulated to prevent or reverse emphysema, however, this has not been examined in the rat model of elastase-induced emphysema. MATERIALS AND METHODS: In this study, 31 Wistar albino rats aged 6–8 weeks and weighing 250–300 g were assessed. On day 1, the animals were treated intratracheally with 0.5 mL saline (control group, n=10), i.e., 0.5 mL saline solution containing 0.1 IU porcine pancreatic elastase (PPE) (Elastase group, n=12) or PPE plus MSC (Elastase-MSC group, n=9) was adminstered per animal. MSCs suspended in serum were injected via the caudal vein on day 21. At least 106 cells were injected. All animals were sacrificed on day 42 and the emphysema index (EI) was calculated, along with measuring the BALF and serum MMP-9 concentrations. RESULTS: Porcine pancreatic elastase induced a significant degree of emphysema in the PPE groups as compared to the control group, which was determined by the EI index (p=0.008). This was not reversed by MSC treatment. The EI remained significantly low in comprison with the controls (p=0.001) and measured no different from the Elastase-treated animals. There was no statistically significant difference between the BALF and serum MMP-9 levels between the control and treatment groups. CONCLUSION: Our findings suggest that therapeutic treatment with adipose tissue-derived MSC in rats has no effect on emphysema or on MMP9 expression, which is a known marker of emphysema.
2020-01-01T00:00:00ZOutcomes of Eltrombopag Treatment and Development of Iron Deficiency in Children with Immune Thrombocytopenia in Turkey
https://hdl.handle.net/20.500.12587/13964
Outcomes of Eltrombopag Treatment and Development of Iron Deficiency in Children with Immune Thrombocytopenia in Turkey
Yozgat, Ayça Koca; Yaralı, Neşe; Özbek, Namık Yaşar; Leblebisatan, Göksel; Şaşmaz, Hatice İlgen; Akbayram, Sinan; Özel, Simge Çınar
Objective: Immune thrombocytopenia (ITP) is a rare autoimmune disease and hematologic disorder characterized by reduced platelet counts that can result in significant symptoms, such as bleeding, bruising, epistaxis, or petechiae. The thrombopoietin receptor agonist eltrombopag (EPAG) is a second-line agent used to treat chronic ITP purpura in adults and children. Materials and Methods: The present retrospective study evaluated the efficacy, safety, and side effects of EPAG treatment in pediatric patients with acute refractory and chronic immune thrombocytopenia, particularly focusing on iron-deficiency anemia. Results: The diagnosis was chronic ITP in 89 patients and acute refractory ITP in 16 patients. The mean age of patients was 9.5±4.5 years (minimum-maximum: 1.2-18 years) at the beginning of EPAG treatment. The overall response rate was 74.3% (n=78). The mean time for platelet count of ?50x109/L was 11.6±8 weeks (range: 1-34 weeks). The treatment was stopped for 27 patients (25.7%) at an average of 6.8±9 months (range: 1-38 months). The reason for discontinuation was lack of response in 18 patients, nonadherence in 4 patients, and hepatotoxicity in 2 patients. Response to treatment continued for an average of 4 months after cessation of EPAG in 3 patients.Conclusion: Results of the current study imply that EPAG is an effective therapeutic option in pediatric patients with acute refractory and chronic ITP. However, patients must be closely monitored for response and side effects during treatment, and especially for iron deficiency.
2020-01-01T00:00:00ZEffects of a novel biodegredable implant system on a rat tibia fracture model
https://hdl.handle.net/20.500.12587/13972
Effects of a novel biodegredable implant system on a rat tibia fracture model
Yalçınozan, Mehmet; Türker, Mehmet; Çırpar, Meriç
Objective: This study aimed to determine the effects of a novel biodegradable implant releasing platelet-derived growth factor (PDGF) at the fracture site on fracture healing in a rat tibia fracture model. Methods: In this study, 35 male Sprague-Dawley rats weighing between 300 and 350g were used. The rats were divided into four groups: Group A (control group without any treatment, n=10), Group B (spacer without PDGF Group, n=10), Group C (spacer with PDGF group, n=10), and Group D (healthy rat Group, n=5). Standardized fractures were created in the right tibias of rats, and then biodegradable implants made of poly-?-hydroxybutyrate-co-3-hydroxy valerate were implanted at the fracture sites in Groups B and C. In Group C, implants were loaded with 600 ng of PDGF. Animals were sacrificed 30 days after the operation, and fracture healing in each group was assessed radiologically based on the Goldberg score. Furthermore, the anteroposterior (AP) and mediolateral (ML) callus diameters were measured macroscopically, and fracture sites were mechanically tested. Results: In the radiological assessment, Group C showed higher fracture healing rate than Groups A and B (p=0.001), whereas no significant difference was found between group C and Group D (p>0.05). In the macroscopic assessment, while Group C exhibited the thickest AP callus diameter (p=0.02), no significant differences in ML callus diameters existed among the groups (p>0.05). Mechanical testing revealed that Group C had higher torsional strength (p=0.001) and stiffness than Groups A and B (p=0.001) while there was no significant difference between Groups C and D (p>0.05).
2020-01-01T00:00:00Z