Molecular analysis of three local silkworm breeds (Alaca, Bursa Beyazi and Hatay Sarisi) by RAPD-PCR and SDS-PAGE methods

Abstract

In this study, PCR-based RAPD method was used to determine the genetic variation in three Turkish local silkworm breeds (Alaca, Bursa Beyazı and Hatay Sarısı) and SDS-PAGE method was used to analyse the sericin proteins of their cocoons. Three local breeds were analysed by randomly chosen 40 primers and 68 total RAPD bands observed with 7 of them. Percentages of polymorphic loci were determined higher in Hatay Sarısı (55.88), lower in Bursa Beyazı and Alaca (44.12). Nei's genetic distance was determined 0.0637 between Bursa Beyazı and Alaca, 0.1012 between Bursa Beyazı and Hatay Sarısı, 0.0793 between Alaca and Hatay Sarısı. In SDS-PAGE sericin proteins of three breeds was yielded a single band of 200 kDa.