The investigation of chicken anemia virus and its antibody existence in commercial broiler and broiler

Abstract

The goals of this study were to investigate the antibody existence of chicken anemia virus (CAV) in vaccinated and non-vaccinated broiler breeder flocks and to monitor the course of CAV maternal antibody, to investigate the existence of anti-CAV antibody, CAV VP1 and VP2 genes in commercial broiler flocks which were the progenies of broiler breeder flocks. Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) were used in the study. Between June 2008 and February 2009, 218 blood serum samples were obtained from 6 vaccinated and 6 non-vaccinated broiler breeder flocks with different ages. A total of 2074 serum and 1916 tissues samples were also obtained from 32 commercial broiler flocks. The vaccinated and non-vaccinated broiler breeder flocks were CAV seropositive and mean antibody titers of vaccinated and non-vaccinated broiler breeder flocks were not statistically different. In commercial broiler flocks, the rates of maternal antibodies were detected between 98 - 100%, 5 - 60%, and 0 - 15% in flocks with a day and a week old, with two weeks old, and with three weeks old, respectively. The anti-CAV antibody existence were detected at the rate of 62.5 and 93.8% in 8 commercial broiler flocks with four weeks old and 16 commercial broiler flocks with five weeks old of vaccinated broiler breeder flocks, respectively. CAV VP1 and VP2 genes were detected in 25% of three weeks old, 37.5% four weeks old flocks of 8 commercial broilers and in 37.5% of five weeks old flocks of 16 commercial broilers of vaccinated broiler breeder flocks. However, no CAV antibody, CAV VP1 and VP2 genes were detected in 8 commercial broiler flocks of non-vaccinated broiler breeder flocks.