dc.contributor.author | Denizli, A | |
dc.contributor.author | Senel, S | |
dc.contributor.author | Arica, MY | |
dc.date.accessioned | 2020-06-25T17:34:36Z | |
dc.date.available | 2020-06-25T17:34:36Z | |
dc.date.issued | 1998 | |
dc.identifier.citation | closedAccess | en_US |
dc.identifier.issn | 0927-7765 | |
dc.identifier.uri | https://doi.org/10.1016/S0927-7765(98)00027-7 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12587/2799 | |
dc.description | WOS: 000075540900001 | en_US |
dc.description.abstract | Lysozyme adsorption onto Cibacron Blue F3GA and Cu(II) derived poly(2-hydroxyethyl methacrylate) [poly(HEMA)] membranes was investigated. Microporous poly(HEMA) membranes were prepared by photopolymerization of HEMA. The triazine dye, Cibacron Blue F3GA was attached covalently as a dye-ligand. These dye-membranes with a swelling ratio of 58% (w/w), and carrying different amounts of Cibacron Blue F3GA (between 0.35 and 1.07 mu mol cm(-2)) were used in the lysozyme adsorption studies. The effect of initial concentration and pH on the adsorption efficiency of dye-derived and metal-chelated membranes were studied in a batch reactor. The effect of Cu(II) incorporation on lysozyme adsorption was also studied. The non-specific adsorption of lysozyme on the poly(HEMA) membranes was 0.9 mu g cm(-2). Cibacron Blue F3GA attachment significantly increased the lysozyme adsorption up to 133.3 mu gcm(-2) Lysozyme adsorption capacity of the Cu(II) incorporated membranes (165.1 mu g cm(-2)) was greater than that of the Cibacron Blue F3GA-attached membranes. More than 85% of the adsorbed lysozyme was desorbed in 1 h in the desorption medium containing 0.5 M potassium thiocyanate (KSCN) at pH 8.0. (C) 1998 Elsevier Science B.V. All rights reserved. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier Science Bv | en_US |
dc.relation.isversionof | 10.1016/S0927-7765(98)00027-7 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | dye affinity membranes | en_US |
dc.subject | lysozyme adsorption | en_US |
dc.subject | poly(HEMA) | en_US |
dc.subject | Cibacron Blue F3GA | en_US |
dc.subject | metal chelates | en_US |
dc.title | Cibacron Blue F3GA and Cu(II) derived poly(2-hydroxyethylmethacrylate) membranes for lysozyme adsorption | en_US |
dc.type | article | en_US |
dc.contributor.department | Kırıkkale Üniversitesi | en_US |
dc.identifier.volume | 11 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.startpage | 113 | en_US |
dc.identifier.endpage | 122 | en_US |
dc.relation.journal | Colloids And Surfaces B-Biointerfaces | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |