dc.contributor.author | Arica, MY | |
dc.contributor.author | Alaeddinoglu, NG | |
dc.contributor.author | Hasirci, V | |
dc.date.accessioned | 2020-06-25T17:34:37Z | |
dc.date.available | 2020-06-25T17:34:37Z | |
dc.date.issued | 1998 | |
dc.identifier.citation | closedAccess | en_US |
dc.identifier.issn | 0141-0229 | |
dc.identifier.uri | https://doi.org/10.1016/S0141-0229(97)00139-7 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12587/2806 | |
dc.description | WOS: 000071652100003 | en_US |
dc.description.abstract | Glucoamylase was covalently immobilized onto pHEMA/EGDMA microspheres of two different sizes: 50-100 mu m and 100-200 mu m in diameter. The activity of the enzyme on smaller microspheres was found to be almost twice that of the larger microspheres. A higher enzyme lending was observed on small microspheres (0.64 mg g(-1) support) as compared to large spheres (0.40 mg g(-1) support). The K-m of glucoamylase was significantly increased (approximately five times) upon immobilization, indicating decreased affinity by the enzyme for its substrate. V-max of the enzyme was, however, not as significantly altered upon immobilization as the K-m. More significantly, the V-max was much higher with the large substrate (dextrin) than it was with the small substrate (maltose). Activity of the immobilized enzyme was quite stable. In 120 h, only 9.0% of the immobilized glucoamylase activity was lost. (C) 1998 Elsevier Science Inc. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier Science Inc | en_US |
dc.relation.isversionof | 10.1016/S0141-0229(97)00139-7 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | pHEMA/EGDMA microspheres | en_US |
dc.subject | covalent bonding | en_US |
dc.subject | enzyme immobilization | en_US |
dc.subject | glucoamylase | en_US |
dc.subject | enzyme reactor | en_US |
dc.title | Immobilization of glucoamylase onto activated pHEMA/EGDMA microspheres: properties and application to a packed-bed reactor | en_US |
dc.type | article | en_US |
dc.contributor.department | Kırıkkale Üniversitesi | en_US |
dc.identifier.volume | 22 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.startpage | 152 | en_US |
dc.identifier.endpage | 157 | en_US |
dc.relation.journal | Enzyme And Microbial Technology | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |