dc.contributor.author | Arikan, S | |
dc.contributor.author | Sands, HS | |
dc.contributor.author | Rodway, RG | |
dc.contributor.author | Batchelder, DN | |
dc.date.accessioned | 2020-06-25T17:35:01Z | |
dc.date.available | 2020-06-25T17:35:01Z | |
dc.date.issued | 2002 | |
dc.identifier.issn | 0378-4320 | |
dc.identifier.uri | https://doi.org/10.1016/S0378-4320(02)00020-9 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12587/2995 | |
dc.description | WOS: 000176451100010 | en_US |
dc.description | PubMed: 12047933 | en_US |
dc.description.abstract | Raman spectroscopy has been used to identify and locate beta-carotene within individual living luteal cells. The cells were either freshly prepared or cultured; the latter was incubated in the presence or absence of beta-carotene in the form of enriched bovine high-density lipoprotem. Luteal cells were investigated using several Raman spectroscopic and imaging techniques. These techniques did not give accurate concentration levels of beta-carotene within parts of the cell but illustrated the distribution of the molecule. Freshly prepared luteal cells were found to contain an appreciable concentration of beta-carotene. Over a period of several days, the concentration gradually reduced to a nearly undetectable level; similar results were found for cells cultured in the absence of the beta-carotene. For cells cultured in the presence of beta-carotene, the molecular concentration was maintained for as long as 2 weeks. The Raman spectra of fragmented cells showed that the beta-carotene is predominantly localised in the lipid-rich cell components, with the concentration highest in the microsomal fraction. The Raman imaging techniques revealed that beta-carotene was spread over the entire volume of the luteal cells with higher levels occurring at distinct sites, including the surface. (C) 2002 Elsevier Science B.V. All rights reserved. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier Science Bv | en_US |
dc.relation.isversionof | 10.1016/S0378-4320(02)00020-9 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | cattle-ovary | en_US |
dc.subject | luteal cells | en_US |
dc.subject | carotene | en_US |
dc.subject | Raman spectroscopy | en_US |
dc.title | Raman spectroscopy and imaging of beta-carotene in live corpus luteum cells | en_US |
dc.type | article | en_US |
dc.contributor.department | Kırıkkale Üniversitesi | en_US |
dc.identifier.volume | 71 | en_US |
dc.identifier.issue | 3-4 | en_US |
dc.identifier.startpage | 249 | en_US |
dc.identifier.endpage | 266 | en_US |
dc.relation.journal | Animal Reproduction Science | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |