dc.contributor.author | Ozkara, S | |
dc.contributor.author | Yavuz, H | |
dc.contributor.author | Patir, S | |
dc.contributor.author | Arica, MY | |
dc.contributor.author | Denizli, A | |
dc.date.accessioned | 2020-06-25T17:35:12Z | |
dc.date.available | 2020-06-25T17:35:12Z | |
dc.date.issued | 2002 | |
dc.identifier.issn | 0149-6395 | |
dc.identifier.uri | https://doi.org/10.1081/SS-120001456 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12587/3052 | |
dc.description | WOS: 000174241300013 | en_US |
dc.description.abstract | The pseudo-biospecific affinity ligand L-histidine immobilized poly(2-hydroxyethylmethacrylate) (PHEMA) in spherical form (100-150 mum in diameter) was used for the affinity chromatographic separation of human-immunoglobulin-G (HIgG) from aqueous solutions and human plasma. The PHEMA adsorbents were prepared by a radical suspension polymerization technique. Reactive aminoacid-ligand L-histidine was then immobilized by covalent binding onto these adsorbents. Elemental analysis of immobilized L-histidine for nitrogen was estimated as 62.3 mg L-histidine/g of PHEMA. The maximum HIgG adsorption on the L-histidine immobilized PHEMA adsorbents was observed at pH 7.4. The non-specific HIaG adsorption onto the plain PHEMA adsorbents was very low- (about 0.167 mg/g). Higher adsorption values (up to 3.5 mg/g) were obtained when the L-histidine immobilized PHEMA adsorbents were used from aqueous solutions. Much higher amounts of HIgG were adsorbed from human plasma (up to 44.8 mg/g). Adsorption capacities of other blood proteins were obtained as 2.2 mg/g for fibrinogen and 2.8 mg/g for albumin. The total protein adsorption was determined as 52.1 mg/g. The affinity microbeads allowed the one-step separation of HIgG from human plasma. The HIgG molecules could be repeatedly adsorbed and desorbed with these L-histidine-immobilized PHEMA adsorbents without noticeable loss in their HIgG adsorption capacity. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Marcel Dekker Inc | en_US |
dc.relation.isversionof | 10.1081/SS-120001456 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.title | Separation of human-immunoglobulin-G from human plasma with L-histidine immobilized pseudo-specific bioaffinity adsorbents | en_US |
dc.type | article | en_US |
dc.contributor.department | Kırıkkale Üniversitesi | en_US |
dc.identifier.volume | 37 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.startpage | 717 | en_US |
dc.identifier.endpage | 731 | en_US |
dc.relation.journal | Separation Science And Technology | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |