Preparation of methacrylamide grafted and dye-ligand immobilized PET fibers: Studies of adsorption and purification of lysozyme
dc.contributor.author | Karakisla, Meral | |
dc.contributor.author | Bayramoglu, Guelay | |
dc.contributor.author | Arica, M. Yakup | |
dc.date.accessioned | 2020-06-25T17:44:32Z | |
dc.date.available | 2020-06-25T17:44:32Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | closedAccess | en_US |
dc.identifier.issn | 0021-8995 | |
dc.identifier.uri | https://doi.org/10.1002/app.27698 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12587/4135 | |
dc.description | WOS: 000255043100069 | en_US |
dc.description.abstract | In this study, novel affinity chromatographic fibers was prepared from methacrylamide grafted poly(ethylene terephthalate), PET-g-pMAA, using benzoyl-peroxide as an initiator. A dye ligand (i.e., Procion Brown) as a ligand was then covalently immobilized on the different amount of pMAAm grafted PET fibers, (PET-g-pMAAm-PB). The fibers were characterized by surface area measurement, infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). Adsorptive properties of the composite fibers were tested using a model protein (i.e., lysozyme). To achieve these purposes, the influence of pH, ionic strength, initial lysozyme concentration, and temperature on adsorption system has been investigated and evaluated. A maximum lysozyme adsorption PET-g-pMAAm-PB fiber was obtained as 43.9 mg g(-1) at pH 7.5. The experimental equilibrium data obtained for lysozyme adsorption onto PET-g-pMAAm-PB fibers fitted well to the Langmuir isotherm model. The result of kinetic analyzed for lysozyme adsorption onto affinity fibers showed that the second-order rate equation was favorable. The purity of the eluted lysozyme, as determined by HPLC, was 84% with recovery 73% for PET-g-pMAAm-PB fiber. Experiments on regeneration and dynamic adsorption were also performed. It appears that PET-g-pMAAm-PB fibers can be applied for lysozyme separation without causing any denaturation. (C) 2008 Wiley Periodicals, Inc. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | John Wiley & Sons Inc | en_US |
dc.relation.isversionof | 10.1002/app.27698 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | PET fibers | en_US |
dc.subject | methacrylamide | en_US |
dc.subject | graft copolymerization | en_US |
dc.subject | dye ligand | en_US |
dc.subject | adsorption | en_US |
dc.subject | lysozyme | en_US |
dc.subject | isotherm model | en_US |
dc.subject | kinetics | en_US |
dc.title | Preparation of methacrylamide grafted and dye-ligand immobilized PET fibers: Studies of adsorption and purification of lysozyme | en_US |
dc.type | article | en_US |
dc.contributor.department | Kırıkkale Üniversitesi | en_US |
dc.identifier.volume | 108 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.startpage | 3313 | en_US |
dc.identifier.endpage | 3323 | en_US |
dc.relation.journal | Journal Of Applied Polymer Science | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
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