Plasma levels of interleukin-10 and nitric oxide in response to two different desflurane anesthesia flow rates

Abstract

Objective: This study investigated interleukin-10 and nitric oxide plasma levels following surgery to determine whether there is a correlation between these two variables and if different desflurane anesthesia flow rates influence nitric oxide and interleukin-10 concentrations in circulation. Materials and methods: Forty patients between 18 and 70 years and ASA I-II physical status who were scheduled to undergo thyroidectomy were enrolled in the study. Interventions: Patients were allocated into two groups to receive two different desflurane anesthesia flow rates: high flow (Group HF) and low flow (Group LF). Measurements: Blood samples were drawn at the beginning (t(0)) and end (t(1)) of the operation and after 24h (t(2)). Plasma interleukin-10 and nitric oxide levels were measured using an enzyme-linked-immunosorbent assay and a Griess reagents kit, respectively. Hemodynamic and respiratory parameters were assessed. Results: There was no statistically significant difference between the two groups with regard to interleukin-10 levels at the times of measurement. Interleukin-10 levels were increased equally in both groups at times t(1) and t(2) compared with preoperative concentrations. For both groups, nitric oxide circulating concentrations were significantly reduced at times t(1) and t(2) compared with preoperative concentrations. However, the nitric oxide value was lower for Group HF compared to Group LF at t(2). No correlation was found between the IL-10 and nitric oxide levels. Conclusion: Clinical usage of two different flow anesthesia forms with desflurane may increase interleukin-10 levels both in Group HF and Group LF; nitric oxide levels circulating concentrations were significantly reduced at times t(1) and t(2) compared with preoperative concentrations; however, at 24h postoperatively they were higher in Group LF compared to Group HF. No correlation was detected between interleukin-10 and nitric oxide levels. (C) 2013 Sociedade Brasileira de Anestesiologia. Published by Elsevier Editora Ltda. All rights reserved.