dc.contributor.author | Malkoc, Meral Arslan | |
dc.contributor.author | Demir, Necla | |
dc.contributor.author | Sengun, Abdulkadir | |
dc.contributor.author | Bozkurt, Serife Buket | |
dc.contributor.author | Hakki, Sema Sezgin | |
dc.date.accessioned | 2020-06-25T18:15:43Z | |
dc.date.available | 2020-06-25T18:15:43Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Malkoç MA, Demİr N, Şengün A, Bozkurt ŞB, Hakki SS. Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using realtime cell analysis. J Adv Prosthodont. 2015 Feb;7(1):21-26. | en_US |
dc.identifier.issn | 2005-7806 | |
dc.identifier.issn | 2005-7814 | |
dc.identifier.uri | https://doi.org/10.4047/jap.2015.7.1.21 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12587/6231 | |
dc.description | SENGUN, Abdulkadir/0000-0002-6053-160X | en_US |
dc.description | WOS: 000350184300004 | en_US |
dc.description | PubMed: 25722833 | en_US |
dc.description.abstract | PURPOSE. To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs). MATERIALS AND METHODS. Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 mu L of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments. RESULTS. All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P<.001). When the cells were exposed to media by Ultratemp, the cell viability was similar to that of the control at 24 hours (P>.05); however, the cell viability was significantly reduced at 72 hours (P<.001). Light and scanning electron microscopy examination confirmed these results. CONCLUSION. The cytotoxic effects of temporary cements on pulpal tissue should be evaluated when choosing cement for luting provisional restorations. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Korean Acad Prosthodontics | en_US |
dc.relation.isversionof | 10.4047/jap.2015.7.1.21 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Cytotoxicity | en_US |
dc.subject | Temporary cements | en_US |
dc.subject | Real-time cell analysis | en_US |
dc.subject | Dental pulp-derived cells | en_US |
dc.title | Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using real-time cell analysis | en_US |
dc.type | article | en_US |
dc.contributor.department | Kırıkkale Üniversitesi | en_US |
dc.identifier.volume | 7 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.startpage | 21 | en_US |
dc.identifier.endpage | 26 | en_US |
dc.relation.journal | Journal Of Advanced Prosthodontics | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |