dc.contributor.author | Azar, Mehdi Tayybi | |
dc.contributor.author | Saglam, Necdet | |
dc.contributor.author | Turk, Mustafa | |
dc.date.accessioned | 2020-06-25T18:34:31Z | |
dc.date.available | 2020-06-25T18:34:31Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | closedAccess | en_US |
dc.identifier.issn | 1662-5250 | |
dc.identifier.issn | 1661-9897 | |
dc.identifier.uri | https://doi.org/10.4028/www.scientific.net/JNanoR.58.1 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12587/7940 | |
dc.description | WOS: 000472033600001 | en_US |
dc.description.abstract | Wnt-beta Catenin Pathway has an important role in many cancers. Wnt-1 protein from Wnt protein family that regulate this pathway has a special effect on the development of breast cancer. Monoclonal antibodies attach to metal nanoparticles have an important role in diagnosis and treatment of cancers. In this study, Anti Wnt-1 monoclonal antibody was conjugated to the gold nanoparticles synthesized by Turkevich method. Conjugation was achieved using EDC-NHS method. The density of the monoclonal antibodies that bonded to gold nanoparticles was measured by Roche Cobas Integra 400 Plus device. MCF-7 breast cancer cell line was treated with conjugated nanoparticles for 48 h then Double Staining method was used to detect apoptosis cells. The results showed that inhibition of Wnt-1 protein in extracellular matrix causes apoptosis and gold nanoparticles have a positive effect on Anti Wnt-1 monoclonal antibodies and this positive effect causes apoptosis to increase rate in conjugating nanoparticles. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Trans Tech Publications Ltd | en_US |
dc.relation.isversionof | 10.4028/www.scientific.net/JNanoR.58.1 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Epithelial mesenchymal transition (EMT) | en_US |
dc.subject | WNT/beta-catenin pathway | en_US |
dc.subject | Gold nanoparticles | en_US |
dc.subject | Anti Wnt-1 Monoclonal antibodies | en_US |
dc.subject | MCF-7 Breast Cancer cell lines | en_US |
dc.subject | Apoptosis | en_US |
dc.title | Anti Wnt-1 Monoclonal Antibody's Conjugated with Gold Nanoparticles, Induced Apoptosis on MCF-7 Breast Cancer Cell Lines | en_US |
dc.type | article | en_US |
dc.contributor.department | Kırıkkale Üniversitesi | en_US |
dc.identifier.volume | 58 | en_US |
dc.identifier.startpage | 1 | en_US |
dc.identifier.endpage | 9 | en_US |
dc.relation.journal | Journal Of Nano Research | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |