The usefulness of GST lsoenzymes as markers of malignancy in urinary cytology

Abstract

This study investigated the usefulness of GST lsoenzymes immunostaining as an early marker of malignancy in urinary cytology. They are also correlated with cytopathology diagnosis. In this study, the slides from 22 patients of benign urinary cytology who have benign urinary diseases and 6 patients who have clinical diagnosis of bladder carcinoma were observed. The cytomorphological characteristics and the immunocytochemical expression of GST alpha (GSTA), pi (GSTP), mu (GSTM4), theta (GSTT 1) isoen2ymes were examined. Immunohistochemical examinations showed that the urothelial cancer cells had stronger staining intensity than the benign cells in 5 (83%) cases for GSTA, 5 (%83) cases for GSTP, 1(%17) case for GSTM4 and 4 (%67) cases for GSTT1. The atypical cells had stronger staining intensity than the benign cells in 10 (%45) cases for GSTA, 16 (%73) cases for GSTP, 12 (%55) cases for GSTM4 and 13(%59) cases for GSTT1. There was no significant difference in GSTA, GSTM4, GSTT1, GSTP expressions for benign, atypical and malign cells (p>0.05). In conclusion, GST lsoenzymes were not found to be markers of malignancy in urinary cytology. © IDOSI Publications, 2010.