Production of high temperature-resistant strains of Agaricus bitorquis

Abstract

In this study, the culture mushroom Agaricus bitorquis (Quel.) Sacc. was examined for growth of mycelia and fructifications under high temperature. The spores taken from the mushrooms that were collected from nature were grouped as A, B, C, D and E. These spores were inoculated into malt extract agar and incubated at 30 degrees C and primer mycelium was produced. The mycelium discus taken from primer mycelium in 8 mm diameter were inoculated into the center of malt extract agar and incubated at 30 degrees C, 32 degrees C, 34 degrees C, 36 degrees C, and 38 degrees C, separately. During the incubation period the growth of the mycelia were measured. The best mycelia growth for all groups was seen at 30 degrees C. At 36 degrees C, the E group mycelia and at 38 degrees C other group's mycelia did not grow. These temperatures were determined as thermal lethal point for the groups. From all the mycelia produced spawn was prepared and inoculated into compost and incubated at 30 degrees C and 32 degrees C. The harvested mushrooms were inspected morphologically.