Rapid detection of Listeria monocytogenes in chicken carcasses by IMS-PCR
Date
2009Author
Ayaz, Naim DenizAyaz, Yildiz
Kaplan, Yusuf Ziya
Dogru, Aylin Kasimoglu
Aksoy, Mihriban Hatun
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The aim of this study was to determine the prevalence and seasonal distribution of Listeria, monocytogenes as determined in packaged, fresh chicken carcasses purchased from supermarkets in Ankara, Turkey. Immunomagnetic separation (IMS) and polymerase chain reaction (PCR) assay were performed, based on hlyA gene (listeriolysin O [LLO]) specific primers. A total of 240 chicken carcasses were investigated over a one-year period. Forty-nine (20.2%) of the samples were determined as L. monocytogenes positive. It was found that the seasonal effect was not a significant factor for the prevalence of L. monocytogenes in chicken carcasses.