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dc.contributor.authorAkturk, Omer
dc.contributor.authorKismet, Kemal
dc.contributor.authorYasti, Ahmet C.
dc.contributor.authorKuru, Serdar
dc.contributor.authorDuymus, Mehmet E.
dc.contributor.authorKaya, Feridun
dc.contributor.authorKeskin, Dilek
dc.date.accessioned2020-06-25T18:22:30Z
dc.date.available2020-06-25T18:22:30Z
dc.date.issued2016
dc.identifier.citationclosedAccessen_US
dc.identifier.issn2046-2069
dc.identifier.urihttps://doi.org/10.1039/c5ra24225h
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6773
dc.descriptionWOS: 000369546100056en_US
dc.description.abstractThis study aimed to fabricate 3D silk fibroin (SF) matrices for skin tissue engineering applications. SF/poly(ethylene oxide) solutions were wet electrospun to obtain a fibrous network (0.7-20 mm diameter), which were then lyophilized to obtain 3D porous nanofibrous matrices (SFM-E: ethanol treated silk fibroin matrices). SF matrices were loaded with citrate-capped gold nanoparticles (AuNPs, 14.27 ppm, D-average = 24 nm) (SFM-AuE: ethanol treated silk fibroin matrices incorporated with AuNPs) and investigated for structural and chemical properties, in vitro biocompatibility and in vivo full-thickness dermal wound healing efficacy in a rat model. AuNP incorporation enhanced the degradation profiles and mechanical properties significantly. SFM-E and SFM-AuE showed similar cell attachment and layer by layer proliferation behaviour, but cells had more spread and flattened morphology on SFM-AuE. Both matrix extracts had high cell viability (>90%), indicating good in vitro biocompatibility. Wound closure was statistically more than the untreated skin control (UTSC) in SFM-E and SFM-AuE applied groups. The recovered tensile strength and elastic modulus of SFM-E and SFM-AuE (40-60%) were not as high as the unwounded skin control (UWSC), but they had elongation at break values similar to UWSC. This was attributed to the still ongoing medium to high inflammation levels leading to a low and immature extent of collagen fibrils on postoperative 14th day. There was only a small amount of epithelialization due to scab formation and medium to high level inflammation for both SFM-E and SFM-AuE, but they were better than UTSC in terms of neovascularization and granulation tissue formation. As a whole, inclusion of AuNPs to SF matrices at 14.27 ppm loading brought some enhancement in the matrix properties and did not cause any toxicity in in vitro and in vivo conditions and even had potency to promote wound healing stages.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey, TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [2120189]en_US
dc.description.sponsorshipThis investigation was financially supported by the Scientific and Technological Research Council of Turkey, TUBITAK (Project no. 2120189) in the framework of 1512-Entrepreneurship and Multistep R&D Funding Program. The commercialization potential of the SF matrices developed here was also investigated with the cooperation of METU and Remoderm Medical Ltd. Co. The authors express their gratitude to Tufan Emiroglu for his helps in construction of wet electrospinning system and Dr Temel Bilici for his valuable advices in the design of SF matrices. The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.en_US
dc.language.isoengen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.isversionof10.1039/c5ra24225hen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleWet electrospun silk fibroin/gold nanoparticle 3D matrices for wound healing applicationsen_US
dc.typearticleen_US
dc.contributor.departmentKırıkkale Üniversitesien_US
dc.identifier.volume6en_US
dc.identifier.issue16en_US
dc.identifier.startpage13234en_US
dc.identifier.endpage13250en_US
dc.relation.journalRsc Advancesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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