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dc.contributor.authorKilic, Nil
dc.contributor.authorDerici, Mehmet Kursat
dc.contributor.authorBuyuk, Ilker
dc.contributor.authorAydin, Semra Soydam
dc.contributor.authorAras, Sumer
dc.contributor.authorCansaran-Duman, Demet
dc.date.accessioned2020-06-25T18:29:23Z
dc.date.available2020-06-25T18:29:23Z
dc.date.issued2018
dc.identifier.citationKilic, N., Derici, M. K., Büyük, İ., Aydin, S. S., Aras, S., & Cansaran Duman, D., (2018). Evaluation of in vitro Anticancer Activity of Vulpinic Acid and its Apoptotic Potential Using Gene Expression and Protein Analysis. Indıan Journal Of Pharmaceutıcal Educatıon And Research , vol.52, no.4, 626-634.en_US
dc.identifier.issn0019-5464
dc.identifier.urihttps://doi.org/10.5530/ijper.52.4.73
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7284
dc.descriptionDerici, Mehmet Kursat/0000-0002-8260-7492; Cansaran-Duman, Demet/0000-0003-3759-6664; Cansaran Duman, Demet/0000-0001-5662-2333en_US
dc.descriptionWOS: 000450531500011en_US
dc.description.abstractLichens and their secondary metabolite are still among the many unexamined natural sources in the drug industry. This study was designed to evaluate the cytotoxic effects of vulpinic acid lichen secondary metabolite and 6.25, 12.5, 25, 50, 100, 200 and 400 mu M concentrations that treat cancer cell lines (CaCo2, HepG2, Hep2C, RD Wehi) and normal cells (Vero and L929) by MTT assay. The aim of this study was to determine the apoptotic effect of vulpinic acid on a molecular level. The determination of apoptotic molecular patterns of vulpinic acid was performed on western blot and quantitative real-time PCR (qRT-PCR) analysis. In our study, transcriptome changes on both gene and protein levels showed similar results. The determination of, mRNA levels of Bax, Bcl-2 and P53 genes were showed by qRT-PCR in cancer and normal cell lines. The results of the study showed that IC50 value of vulpinic acid altered the mRNA levels of Bax, Bcl-2 and P53 genes in all examined cancer cells when compared to the untreated cells. When the mRNA levels of the examined genes were compared, it was observed that Box gene showed more expression increase in all cell lines when compared to Bcl-2 and P53 genes. This is the first evaluation of the apoptotic effect of vulpinic acid secondary metabolite on mRNA levels. The current study highlights some points regarding vulpinic acid and its use for cancer treatment.en_US
dc.description.sponsorshipAnkara University Project OfficesAnkara University [16H0415002, 15B0415001]en_US
dc.description.sponsorshipWe thank Ankara University Project Offices, Project no. 16H0415002 and Project no. 15B0415001 for the partly financial support.en_US
dc.language.isoengen_US
dc.publisherAssoc Pharmaceutical Teachers Indiaen_US
dc.relation.isversionof10.5530/ijper.52.4.73en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectVulpinic aciden_US
dc.subjectApoptotic effecten_US
dc.subjectqRT-PCRen_US
dc.subjectWestern bloten_US
dc.titleEvaluation of in vitro Anticancer Activity of Vulpinic Acid and its Apoptotic Potential Using Gene Expression and Protein Analysisen_US
dc.typearticleen_US
dc.contributor.departmentKırıkkale Üniversitesien_US
dc.identifier.volume52en_US
dc.identifier.issue4en_US
dc.identifier.startpage626en_US
dc.identifier.endpage634en_US
dc.relation.journalIndian Journal Of Pharmaceutical Education And Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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