Simsek, OzkanMihm, Monika2020-06-252020-06-252014Şimşek, Ö. and Mihm, M. (2014) "Activity of 3\beta-hydroxysteroid dehydrogenase associated with progesterone production in bovine granulosa cells cultured under different concentrations of serum, insulin-like growth factor I, and gonadotropin," Turkish Journal of Veterinary & Animal Sciences. 38(4),358-362.1300-0128https://doi.org/10.3906/vet-1311-57https://hdl.handle.net/20.500.12587/5920Three-beta-hydroxysteroid dehydrogenase (3 beta-HSD) is the enzyme responsible for progesterone production. This study aimed to determine whether 3 beta-HSD activity can be shown to reflect progesterone production by granulosa cells cultured under different serum conditions and follicle stimulating hormone (FSH), luteinising hormone (LH), and insulin-like growth factor I (IGF-I) concentrations. Large bovine follicles were dissected from abattoir ovaries to recover granulosa cells. Cells were washed, stained for viability, and plated for 48 h in basic medium with or without 5% foetal calf serum (FCS). Subsequently, cells were exposed to FSH (1 ng/mL), LH (10 or 100 ng/mL), or FSH (1 ng/mL) + IGF-I (1 or 10 ng/mL) in a serum-free medium for another 96 h to predict degree of luteinisation. Before and after incubation, granulosa cells were stained for 3 beta-HSD activity. The high dose of IGF-I (10 ng/mL) increased (P < 0.05) progesterone secretion over 2.5-fold compared with FSH alone or the low dose of IGF-I (1 ng/mL) in cells preincubated with FCS. This was clearly reflected by darker 3 beta-HSD staining than in cells exposed to FSH and low dose IGF-I.eninfo:eu-repo/semantics/openAccess3 beta-Hydroxysteroid dehydrogenasebovine granulosa cellsprogesteroneFSHLHIGF-IArticle38435836210.3906/vet-1311-572-s2.0-84902284746Q3WOS:000338320300003Q4