Ağalar, CananÖzdoğan, MehmetAğalar, FatihSaygun, OralAydınuraz, KuzeyAkkuş, AbdullahAktürk, Selçuk2020-06-252020-06-252006closedAccess1445-1433https://doi.org10.1111/j.1445-2197.2006.03728.xhttps://hdl.handle.net/20.500.12587/3791Background: The aim of this study was to constitute a valid graft infection model with Staphylococcus epidermidis in rats. Method: Rats were divided into seven groups. In groups 1 and 2, 2 cm x 2 cm polypropylene grafts were incubated with 10(8) c.f.u./mL slime-positive S. epidermidis at 37 degrees C for 2 and 24 h and were then placed subfascially to the groins of rats. In the third group, naive grafts were placed and 0.5 mL of 3 x 10(7) c.f.u. slime-positive S. epidermidis were injected on the inside of the wounds. Rifampicin (30 mg/kg) in group 4 and teicoplanin (20 mg/kg) in group 5 were applied i.p. to rats with 2-h incubated grafts for prophylaxis. The same prophylactic regimens were given to groups 6 and 7 in which rats were incubated for 24 h. At eighth day, rats were killed and wounds were assessed with macroscopic evaluation and cultures. Results: No death occurred in any of the groups. In groups 1 and 2, 100% infection rates were achieved. However, graft infection was detected in only two (20%) of the rats in group 3 (P = 0.001). Prophylactic application of teicoplanin or rifampicin decreased the infection rates significantly in the short-incubation groups. Conclusion: Incubation of polypropylene grafts with slime-producing S. epidermidis for 2 and 24 h in the pre-application period achieved the occurrence of a standardized graft infection. Prophylactic use of teicoplanin and rifampicin decreased the infection rates. We propose to use this reproducible and reliable animal model of graft infection in future studies.eninfo:eu-repo/semantics/closedAccessforeign body infectiongraftherniapolypropyleneStaphylococcus epidermidisArticle76538739110.1111/j.1445-2197.2006.03728.x2-s2.0-3374477198216768701Q2WOS:000237738500023Q3