Azkur, Ahmet KursatAksoy, EmelYildirim, MuratYildiz, Kader2021-01-142021-01-142020Azkur AK, Aksoy E, Yildirim M, Yildiz K: Optimisation of indirect ELISA by comparison of different antigen preparations for detection of antibodies against Schmallenberg virus. Kafkas Univ Vet Fak Derg, 26 (6): 795-800, 2020.1300-6045https://doi.org/10.9775/kvfd.2020.24397https://hdl.handle.net/20.500.12587/12538Schmallenberg virus (SBV) infection, discovered in 2011, was reported in Europe including Turkey, Africa and recently in some Asian countries. Commercial enzyme-linked immunosorbent assay (ELISA) kits were widely used by researchers in many epidemiological studies and SBV diagnosis. The aim of this study was to optimise indirect in-house ELISA that is based on different antigen preparations of cell-culture derived whole SBV particle. Antigen preparations were maintained with various methods: PEG precipitation, ultracentrifugation, dialysis, and antigen inactivation. Following antigen optimisation, steps of antigen coating, blocking, conjugate and stop solution were optimised and in-house ELISA was compared to commercial indirect SBV ELISA kit. The best result in ELISA antigen preparation for SBV was gained by 30% PEG purification method followed by formaldehyde inactivation. Although results of this study demonstrated that in-house ELISA for detection of SBV specific antibodies was equally sensitive and specific as commercial kit, purified SBV antigen based in-house ELISA development could increase S/P ratios.eninfo:eu-repo/semantics/openAccessDiagnosisDialysisELISAPEGSchmallenberg virusArticle26679580010.9775/kvfd.2020.243972-s2.0-85093107536Q3420201WOS:000582334700010Q4