Sagsöz, N.Kısa, Ü.Apan, A.2020-06-252020-06-252002closedAccess0268-1161https://doi.org/10.1093/humrep/17.11.2972https://hdl.handle.net/20.500.12587/2966KISA, Ucler/0000-0002-8131-6810BACKGROUND: In this prospective controlled study, the aim was to examine the effects of vitamin C, mannitol and verapamil on adnexial ischaemia-reperfusion injury in the rat ovary. METHODS: Thirty-six female Wistar rats were used. In the controls (group 1), only laparotomy was performed. In group 2, ovarian ischaemia was produced and the bilateral ovaries were surgically removed 4 h later. In group 3, an ischaemic period of 4 h was followed by reperfusion for 1 h; the bilateral ovaries were then removed. In groups 4, 5 and 6, after 4 h of ischaemia, either vitamin C, mannitol or verapamil respectively was infused before reperfusion; after 1 h of reperfusion the ovaries were removed. Thiobarbituric acid reactive substance (TBARS) levels were measured in all ovary tissues. RESULTS: TBARS levels of the reperfusion group were significantly higher than those of groups treated with vitamin C or mannitol (P = 0.013 and P = 0.045 respectively), but not of the verapamil group. CONCLUSIONS: Vitamin C and mannitol were found to be effective in reducing ischaemia-reperfusion injury of the ovary during its early stages, but verapamil was ineffective.eninfo:eu-repo/semantics/closedAccessmannitolovaryreperfusionverapamilvitamin CArticle17112972297610.1093/humrep/17.11.29722-s2.0-003685209212407059Q1WOS:000179305200032Q1