Asmafiliz, NuranKilic, ZeynelCivan, MehmetAvci, OrhanGonder, L. YaseminAcik, LeylaHokelek, Tuncer2020-06-252020-06-252016closedAccess1144-05461369-9261https://doi.org/10.1039/c6nj02052fhttps://hdl.handle.net/20.500.12587/6697Hokelek, Tuncer/0000-0002-8602-4382; Aydin, Betul/0000-0002-9092-1350; Asmafiliz, Nuran/0000-0002-9335-4101The condensation reactions of hexachlorocyclotriphosphazene (N3P3Cl6, trimer) with the symmetric N2N2 or N2O2 donor type tetradentate bulky ligands (1-4) gave partly substituted spiro-bino-spiro (sbs) (5-8) trimeric phosphazenes. Compounds 5-8 reacted with pyrrolidine, morpholine and 1,4-dioxa-8-azaspiro[4,5]decane (DASD) to give octapyrrolidino-(9-12), morpholino-(13-16) and DASD-substituted cyclotriphosphazenes (17-20). The structures of the phosphazenes have been elucidated using FTIR, MS, H-1, C-13{H-1} and P-31{H-1} NMR, and HSQC spectral data. The molecular and solid-state structures of 5, 6 and 12 were verified by single crystal X-ray diffraction techniques. On the other hand, the ultrathin and highly ordered Langmuir-Blodgett (LB) films of compounds 6, 7, 9 and 12 were also fabricated. The structural characterization of the LB films was made using p-polarized grazing angle (GAIR) and horizontal attenuated total reflectance (HATR) techniques. All the novel phosphazene derivatives were evaluated for antibacterial activities against Gram-positive (G+) and Gram-negative (G-) bacteria and for antifungal activities against yeast strains. In addition, the cytotoxic effects of compounds 9, 13, 15, 16, 19 and 20 were investigated against L929 fibroblast and MDA-MB-231 breast cancer cells. The most active one among these compounds was compound 9 at 6.25 mu g mL(-1) concentration. The interactions between compounds 5-20 and pBR322 plasmid DNA were determined by agarose gel electrophoresis.eninfo:eu-repo/semantics/closedAccessArticle40119609962610.1039/c6nj02052f2-s2.0-84993967418Q2WOS:000387621500073Q2