Duman, SibelKaya, BulentCaf, FatmaKok, OsmanTuzun, Ilhami2025-01-212025-01-2120242211-9264https://doi.org/10.1016/j.algal.2024.103745https://hdl.handle.net/20.500.12587/25077Recently, the single-celled green freshwater microalgae species Chlorella vulgaris has attracted the attention of researchers due to its different usage areas. In particular, research focuses on the technology of obtaining bio-hydrogen with various techniques. This research involves, for the first time, the use of the microalga Chlorella vulgaris as a bio-supporting material for magnetite Fe3O4 3 O 4 nanoparticles (Fe3O4NPs@Chlorella 3 O 4 NPs@ Chlorella vulgaris) ) and the production of hydrogen through catalytic hydrolysis of NaBH4 4 (sodium borohydride, SB) in the presence of the resulting magnetite nanoparticles. Here, detailed kinetic studies were carried out during the SB-hydrolysis by taking magnetite Fe3O4NPs@Chlorella 3 O 4 NPs@ Chlorella vulgaris and SB in varying amounts and at varying temperatures, and the activation energy and lifetime of magnetite Fe3O4NPs@Chlorella 3 O 4 NPs@ Chlorella vulgaris was found to be 23.49 kJ mol-1- 1 and 93,280 mol H2 2 (mol Fe3O4)-1 , 3 O 4 )- 1 , respectively. No change in the chemical and physical structure of the biocatalyst was observed during the hydrolysis of SB, so only detailed characterization of microalgae and magnetite Fe3O4NPs@Chlorella 3 O 4 NPs@ Chlorella vulgaris was performed, and the particle size of the catalyst was calculated as 10.19 +/- 2.17 nm. The results showed that these Fe3O4NPs@Chlorella 3 O 4 NPs@ Chlorella vulgaris , , which can be easily separated magnetically and have high catalytic activity, are a clean and quite surprising catalyst in terms of hydrogen production.eninfo:eu-repo/semantics/closedAccessMagnetite iron-oxide; Chlorella vulgaris; Micro algae; Sodium borohydride; HydrolysisArticle8410.1016/j.algal.2024.1037452-s2.0-85206128018Q1WOS:001337341000001N/A