Elbüken, Çağlar2025-01-212025-01-2120191308-5514https://dergipark.org.tr/tr/download/article-file/770729https://dergipark.org.tr/tr/pub/umagd/issue/43865/532269https://doi.org/10.29137/umagd.532269https://hdl.handle.net/20.500.12587/20102Identification and successive separation of cellpopulations have a vast number of applications in both clinical research andbasic sciences. In the last few decades several custom-made microfluidicsystems have been developed to address the differentiation needs of theresearchers. For all these systems there is a need for focusing the cells ofinterest in the interrogation zone during the flow. In this article, a newmethod of viscoelastic focusing was used for this purpose. By adding polymericmaterials into the carrying fluid, elastic lift force was generated on thecells that allows passive particle focusing. Once the cells are aligned withthe sensor, detection and identification was achieved using impedancecharacterization. The carrier fluid properties were investigated to achievesuccessful viscoelastic focusing and impedimetric detection. Two blood celltypes, red blood cells and white blood cells, were differentiated based ontheir impedance signal. In addition to its capability as a microfluidic cellcounter, this work reveals the possibility of using viscoelastic focusing forflow through impedance based particle characterization.eninfo:eu-repo/semantics/openAccessMicrofluidicscell countingimpedance detectionviscoelastic focusingArticle12-61261910.29137/umagd.532269532269