Boke, E.Saygi, K. OzlemErenler, R.Kacmaz, B.Ergene, A.2025-01-212025-01-2120240035-001Xhttps://doi.org/10.31349/RevMexFis.70.051001https://hdl.handle.net/20.500.12587/25160This work introduces a systematic and efficient approach for producing stable AgNPs utilizngNigella sativa (Ns) seed aqua extract (AE), which exhibit strong antibacterial properties. The characterization of Ns-AgNPs was performed using a UV-visible spectrophotometer electronic microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDX). The Ns-AgNPs did not show aggregation, as shown by the results of STEM and XRD. According to the EDX analysis in this research, it was determined that Ns-AgNPs, gave signals in the silver region (similar to 3 KeV) at 92.3%. Dynamic light scattering (DLS) was used to determine the average particle size and distribution profile of NsAgNPs. Ns-AgNPs showed significant antibacterial performance againstStaphylococcus aureousand Escherichia coli, being effective at low concentrations. Ns-AgNPs may be incorporated into wound dressings, surgical instruments, and medical devices to prevent infections and promote healing.eninfo:eu-repo/semantics/openAccessSilver nanoparticles; nanotechnology; green synthesis; nigella sativa; antibacterial activity.Article70510.31349/RevMexFis.70.0510012-s2.0-85203004779Q3WOS:001304217800006N/A