Nayci, A.Cakmak, M.Renda, N.Aksoyek, S.Yucesan, S.2020-06-252020-06-252003closedAccess0179-1958https://doi.org/10.1007/s00384-002-0454-zhttps://hdl.handle.net/20.500.12587/3153Nayci, Ali/0000-0002-0534-1205Background and aims: Studies have indicated a deleterious effect of perioperative 5-fluorouracil (5-FU) administration on the healing of intestinal anastomoses. This study examined the effect of early postoperative 5-FU on the healing of colonic anastomoses and investigated the effect of electromagnetic fields (EMF) on colonic anastomotic repair under normal physiological conditions and in the presence of 5-FU therapy in a rat model. Materials and methods: Forty male Wistar rats were randomly assigned into four groups and underwent a standardized left colonic resection and anastomoses. The animals then served as control or received intraperitoneal 5-FU (20 mg/kg per day, 5 days), EMF stimulation (10.76 mT, 50 Hz; 2-h on/10-h off cycles, 7 days) or both, starting on the day of surgery. After 7 days anastomotic healing was assessed by measurement of hydroxyproline content and breaking strength. Results: Hydroxyproline content increased in EMF exposed group (1.53+/-0.11 to 1.92+/-0.11 mug/mg) and in EMF + 5-FU group (1.53+/-0.11 to 1.89+/-0.12 mug/mg). Breaking strength also increased in the EMF group (0.23+/-0.02 to 0.27+/-0.01 MPa) and in the EMF + 5-FU group (0.23+/-0.02 to 0.28+/-0.01 MPa. No differences were found in hydroxyproline content or breaking strength between the 5-FU group and controls. Conclusion: Early postoperative 5-FU administration did not impair the healing of colonic anastomoses in rats. Additionally, EMF stimulation provided a significant gain in colonic anastomotic strength, in rat intestines in control animals and in animals exposed to 5-FU.eninfo:eu-repo/semantics/closedAccessintestineanastomosesmagnetic field5-fluorouracilchemotherapycolonArticle18213614110.1007/s00384-002-0454-z2-s2.0-003805218012548416Q2WOS:000181357100008Q1