Denizli, AArica, Y2020-06-252020-06-252000closedAccess0920-5063https://doi.org/10.1163/156856200743760https://hdl.handle.net/20.500.12587/2873Microporous membranes made of poly(2-hydroxyethylmethacrylate) [poly(HEMA)] carrying protein A were used for selective sorption of human-IgG from human plasma. Poly(HEMA) membranes were prepared by a photo-polymerization technique, and activated by cyanogen bromide (CNBr) in an alkaline medium (pH 11.5). Bioligand protein A was then immobilized by covalent binding onto these CNBr-activated membranes. The amount of immobilized protein A was controlled by changing pH and the initial concentrations of CNBr and protein A. The non-specific adsorption of protein A on the plain poly(HEMA) membranes was 2.9 mu g cm(-2). Maximum protein A immobilization was observed at pH 9.5. Up to 180 mu g cm(-2) was immobilized on the CNBr-activated poly(HEMA) membranes. The maximum adsorption of human-IgG on the protein A-immobilized poly(HEMA) membranes was observed at pH 8.0. The non-specific adsorption of human-IgG onto the plain poly(HEMA) membranes was low (about 4.4 mu g cm(-2)). Higher human-IgG adsorption values (up to 394 mu g cm(-2)) were obtained in which the protein A-immobilized poly(HEMA) membranes were used. Much higher amounts of human-Igg (up to 489 mu g cm(-2)) were adsorbed from human plasma. Up to 91 % of the adsorbed human-IgG was desorbed by using 0.1 M aminoacetic acid as elution agent. The adsorption-desorption cycle was repeated ten times using the same polymeric membranes. There was no remarkable reduction in the adsorption capacity of the protein A-immobilized poly(HEMA) membranes.eninfo:eu-repo/semantics/closedAccessArticle11436738210.1163/1568562007437602-s2.0-003412360710903036Q1WOS:000087993600004Q1