Arıca, M. YakupYılmaz, MeltemBayramoğlu, Gülay2020-06-252020-06-252007closedAccess0021-89951097-4628https://doi.org10.1002/app.25466https://hdl.handle.net/20.500.12587/3946Epoxy group-contain g poly(hydroxyethyl methacrylate/glycidyl methacrylate), p(HEMA/GMA), membrane was prepared by UV initiated photopolymerization. The membrane was grafted with chitosan (CH) and some of them were chelated with Fe(III) ions. The CH grafted, p(HEMA/GMA), and Fe(III) ions incorporated p(HEMA/ GMA)-CH-Fe(III) membranes were used for glucose oxidase (GOD) immobilization via adsorption. The maximum enzyme immobilization capacity of the p(HEMA/GMA)-CH and p(HEMA/GMA)-CH-Fe(HI) membranes were 0.89 and 1.36 mg/mL, respectively. The optimal PH value for the immobilized GOD preparations is found to have shifted 0.5 units to more acidic PH 5.0. Optimum temperature for both immobilized preparations was 10 degrees C higher than that of the free enzyme and was significantly broader at higher temperatures. The apparent K-m values were found to be 6.9 and 5.8 mM for the adsorbed GOD on p(HEMA/GMA)-CH and p(HEMA/GMA)-CH-Fe(HI) membranes, respectively. In addition, all the membranes surfaces were characterized by contact angle measurements. (c) 2006 Wiley Periodicals, Inc.eninfo:eu-repo/semantics/closedAccessmembranecontact angleimmobilized enzymeadsorptionglucose oxidaseArticle10353084309310.1002/app.254662-s2.0-33847421351Q2WOS:000243765100039Q3