Gungor, A. BurakAyaz, YildizKabakli, OzdenCaliskan, ElvinAksoy, MihribanKaplan, Yusuf ZiyaAyaz, Naim Deniz2025-01-212025-01-2120211018-46191610-2304https://hdl.handle.net/20.500.12587/24973Rotaviruses and noroviruses are the main agents that pollute the environment, especially water and food, and cause serious diseases and epidemics in humans by this way. The aims of this study were to compare the efficiency of real time RT-PCR and cell culture techniques for the detection of Rotavirus and Noroviruses and to find out the presence of them in green leafy vegetables and mussels which collected from different markets in Ankara, capital city of Turkey over a 12-month period. Virus positive samples were propagated in MA-104 (ATCC (R) CRL-2378) and Caco2 (ATCC (R) HTB-37 (TM)) cell monolayers, for the infectivity tests. All collected food samples and experimental contaminated foods were analyzed with RT-PCR for detecting Rotavirus and Noroviruses. The results showed that Noroviruses was found to be difficult to reproduce from cell cultures and we were able to produce only Rotavirus isolate on cell lines. As a result of the analysis, it was determined that the titer of the Rotavirus was detected as DKID50 = log10(-4.2) / ml (about 15,000 virus particles / ml). According to the cell culture results; rotavirus was detected in all dilutions in contaminated foods. On the other hand, in samples which were directly analyzed with RT-PCR, the 100 times diluted samples were detected as positive for both viruses. In none of the collected food samples Norovirus and Rotavirus were detected using RT-PCR analysis and cell monolayers. In conclusion, RT-PCR method is useful in detecting rotavirus and noroviruses from foods in routine surveys.eninfo:eu-repo/semantics/closedAccessGreen leafy vegetables; mussels; Norovirus; Rotavirus; RT-PCRArticle30101122811234WOS:000704955000024Q4