Soyer, TutkuSoyer, Ozge UysalBirben, EsraKisa, UclerKalayci, OmerCakmak, Murat2020-06-252020-06-252013closedAccess0022-34681531-5037https://doi.org/10.1016/j.jpedsurg.2013.02.100https://hdl.handle.net/20.500.12587/5433KISA, Ucler/0000-0002-8131-6810; Soyer, Tutku/0000-0003-1505-6042Aim: To evaluate the pepsin and oxidative stress markers in exhaled breath condensate (EBC) in patients with gastroesophageal reflux disease (GERD). Patients and Method: Patients with a presumptive diagnosis of GERD with recurrent respiratory and gastrointestinal problems aged between 2 and 14 years were included in the study. All patients underwent pH monitoring. Patients with a reflux index (RI) >= 4 were assessed as the reflux group, and those with an RO <4 were assessed as the non-reflux group. Pepsin levels and oxidative stress markers [NO metabolites (NOX) and total sulphydrile (TSH) levels] were measured in the EBC. Results: There were 24 patients in the reflux group [RI 17.6 (6.6-46.4)] [median, interquartile range] and 23 in the non-reflux group [RI 0.8 (0.5-1.9) (p < 0.001). Pepsin levels in the EBC were below the level of detection. The median levels of NOx in the EBC of children with reflux [13.7 mu mol/L (7.3-24.5)] were lower in than non-reflux group [21.0 mu mol/L (14.0-25.2)] (p = 0.034). There was a negative correlation between reflux index and NOX levels in EBC (rs: -0.331, p = 0.023). In contrast, there was no difference in TSH levels between the reflux and non-reflux groups [37.4 mu mol/L (30.2-44.6) vs 40.1 mu mol/L (37.4-44.9), respectively, (p > 0.05)]. Conclusion: Decreased levels of NOX in patients with GER disease suggest increased oxidative stress in airways of these patients. (C) 2013 Elsevier Inc. All rights reserved.eninfo:eu-repo/semantics/closedAccessChildrenExhaled breath condensateGastroesophageal reflux diseaseOxidative stressPepsinArticle48112247225010.1016/j.jpedsurg.2013.02.1002-s2.0-8488760414824210194Q1WOS:000327140800012Q3