Erer, D.Dursun, A. D.Oktar, G. L.Iriz, E.Zor, M. H.Elmas, C.Arslan, M.2020-06-252020-06-252014Erer, D., Dursun, A. D., Oktar, G. L., Iriz, E., Zor, M. H., Elmas, Ç., ... Donmez, T.(2014). The effects of iloprost on lung injury induced by skeletal muscle ischemia-reperfusion. BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY, 115(7), 405-410.0006-92481336-0345https://doi.org/10.4149/BLL_2014_080https://hdl.handle.net/20.500.12587/5913Arslan, Mustafa/0000-0003-4882-5063; DURSUN, ALI DOGAN/0000-0001-9056-0025Purpose: The aim of this study was to investigate the effects of iloprost (I) on lung injury as a remote organ following skeletal muscle ischemia-reperfusion injury in a rat model. Materials and methods: Twenty-four Wistar Albino rats were randomized into four groups (n = 6). Laparotomy was performed in all groups under general anesthesia. Only laparotomy was applied in Group S (Sham). Ischemia reperfusion group (Group I/R) underwent ischemia and reperfusion performed by clamping and declamping of the infrarenal abdominal aorta for 120 minutes. Group iloprost (Group 1) received intravenous infusion of iloprost 0.5 ng/kg/min, without ischemia and reperfusion. Group I/R/I received intravenous infusion of iloprost 0.5 ng/kg/min immediately after 2 hours of ischemia. At the end of the study, lung tissue was obtained for determining total oxidant status (TOS) and total antioxidant status (TAS) levels, histochemical and immunohistochemical determination. Results: Diffuse lymphocyte infiltration was detected in immunohistochemical examination of lung tissue in Group I/R. The connective tissue around bronchi, bronchioles and vessel walls was found to be increased. Although minimal local lymphocyte infiltration was detected in some fields in Group I/R/I, the overall tissue was found to be similar to Group S. iNOS expression was significantly higher in Group I/R, when compared with Group S and significantly lower in Group I/R/I compared to Group I/R. TOS levels were significantly higher in Group I/R, when compared with groups S and I (p = 0.028, p = 0.016, respectively) and significantly lower in group I/R/I, when compared with Group I/R (p = 0.048). TAS levels were significantly higher in Group I/R, when compared with groups S, I (p = 0.014, p = 0.027, respectively) and significantly lower in Group I/R/I, when compared with Group I/R (p = 0.032). Conclusion: These results indicate that administration of iloprost may have protective effects against ischemia reperfusion injury (Fig. 8, Tab. 1, Ref. 30). Text in PDF www.elis.sk.eninfo:eu-repo/semantics/openAccessischemia-reperfusiontotal oxidant statustotal antioxidant statusiloprostiNOSlung tissueratArticle115740541010.4149/BLL_2014_0802-s2.0-8490599363325077362Q2WOS:000340863400004Q4