Canayakin, DogukanBayir, YasinBaygutalp, Nurcan KilicKaraoglan, Esen SezenAtmaca, Hasan TarikOzgeris, Fatma Betul KocakHalici, Zekai2020-06-252020-06-252016closedAccess1388-02091744-5116https://doi.org/10.3109/13880209.2016.1145701https://hdl.handle.net/20.500.12587/6704bayir, yasin/0000-0003-3562-6727; ATMACA, HASAN TARIK/0000-0001-8379-4114Context Nigella sativa L. (Ranunculaceae) (NS) is traditionally used to treat many conditions such as inflammation. Objective This study evaluates the effects of NS seeds ethanol extract in paracetamol-induced acute nephrotoxicity in rats. Materials and methods Forty-eight female Wistar Albino rats were divided into eight groups: I = sham; II = sham + 1000 mg/kg NS; III = sham + 140 mg/kg (N-acetyl cysteine) NAC; IV = 2 g/kg paracetamol; V = 2 g/kg paracetamol + 140 mg/kg NAC; VI, VII and VIII = 2 g/kg paracetamol + 250, 500 and 1000 mg/kg NS, respectively. Paracetamol administration (oral) was carried out 1 h after NS and NAC administrations (oral), and all animals were sacrificed 24 h later. Results Paracetamol administration significantly increased serum urea (88.05 U/L) and creatinine (0.80 U/L) when compared with the sham group (49.80 and 0.31 U/L, respectively). However, serum urea level was reduced to 65.60, 56.00 and 54.18 U/L, with 250, 500 and 1000 mg/kg doses of the extract, respectively. Also, serum creatinine level was reduced to 0.64, 0.57 and 0.52 U/L with 250, 500 and 1000 mg/kg doses of the extract, respectively. NS administration increased superoxide dismutase and glutathione, and decreased malondialdehyde levels in the kidneys. Kidney histopathological examinations showed that NS administration antagonized paracetamol-induced kidney pathological damage. Discussion and conclusions The results suggest NS has a significant nephroprotective activity on paracetamol-induced nephrotoxicity. It may be suggested that the antiinflammatory and antioxidant effects of NS ethanolic extract originated from different compounds of its black seeds.eninfo:eu-repo/semantics/closedAccessGlutathionekidneymalondialdehydesuperoxide dismutaseArticle54102082209110.3109/13880209.2016.11457012-s2.0-8498629222726956915Q1WOS:000386041500016Q2