Ekici, SedaBozkaya, EsraBozkaya, OgunCerci, Nebahat AytunaAluc, YasarEkici, Husamettin2025-01-212025-01-2120232365-6549https://doi.org/10.1002/slct.202302102https://hdl.handle.net/20.500.12587/25867This study was aimed to synthesise antimicrobial and therapeutic silver nanoparticles (AgNPs) by green synthesis method using Vitex agnus-castus L. (VAC) seed extract. The synthesised VAC seed extract coated AgNPs were characterised by UV-Vis spectrometry, Zeta analysis and FT-IR spectrometry. The antifungal, antimicrobial, cytotoxicity by MTT assay, and anticarcinogenic activities on HeLa cells line were investigated. VACAgNPs exhibited high antimicrobial activity against S. aureus with a zone diameter of 11.3 +/- 0.6 mm. In addition, the cytotoxicity of VAC-AgNPs was evaluated using the L929 fibroblast cell line and the HeLa cells line, with AgNPs applied at six different concentrations. At a concentration of 5.0 mM, viability was found to be 47.6%+/- 4.2% in L929 fibroblast cells and 39.6%+/- 7.9% in HeLa cells, suggesting a potential antiproliferative effect of VAC-AgNPs on HeLa cancer cells. The IC50 values of VAC-AgNPs against the HeLa cell line were calculated as 2.94 mu g/ml.eninfo:eu-repo/semantics/closedAccessAnticancer; green synthesis; HeLa cells; silver nanoparticles; vitex agnus-castusArticle83210.1002/slct.2023021022-s2.0-85168676355Q3WOS:001062319100007Q3