Bayramoğlu, GülayÖktem, H. AvniArıca, M. Yakup2020-06-252020-06-252007closedAccess1369-703X1873-295Xhttps://doi.org10.1016/j.bej.2006.11.025https://hdl.handle.net/20.500.12587/3904Poly(2-hydroxyethylmethaerylate) (pHEMA) membrane was prepared by photo-polymerization and a dye ligand (i.e., Reactive Green 5) was immobilised on the membrane surface (pHEMA-RG-5). Surface wettability and hydrophilicity of the dye-ligand immobilised membranes were investigated by static contact angle measurements. The chromatographic properties of the dye-ligand immobilized membrane for adsorption and purification of IgG are discussed using bare membrane as a control system. The non-specific adsorption of IgG was low (0.45 mg protein ml(-1) membrane). The attachment of dye ligand onto the membrane significantly increased the IgG adsorption (33.75 mg protein ml(-1) membrane). The maximum IgG adsorption was observed at pH 6.0. The reversible IgG adsorption on the membrane obeyed both the Freundlich and the Langmuir-Freundlich isotherm models. To test the efficiency of IgG isolation from human serum with the pHEMA-RG-5 membrane, the purity of the eluted IgG, as determined by HPLC, was 81% with a recovery of 67%. (c) 2006 Elsevier B.V. All rights reserved.eninfo:eu-repo/semantics/closedAccessadsorptionbiomimeticsmicroporous membranesseparationIgGcontact angleArticle34214715510.1016/j.bej.2006.11.0252-s2.0-33947330124Q2WOS:000245828500007Q1