Denizli, FDenizli, AArica, MY2020-06-252020-06-252000closedAccess0959-8103https://doi.org/10.1002/(SICI)1097-0126(200003)49:3<302https://hdl.handle.net/20.500.12587/2866A new membrane affinity biosorbent carrying thionein has been developed for selective removal of cadmium ions from human serum. Microporous poly(2-hydroxyethyl methacrylate) (pHEMA) membranes were prepared by photopolymerization of HEMA. The pseudo dye ligand Cibacron Blue F3GA (CB) was covalently immobilized on the pHEMA membranes. Then, the cysteine-rich metallopeptide thionein was conjugated onto the CB-immobilized membrane. The maximum amounts of CB immobilized and thionein conjugated on the membranes were 1.07 mu mol cm(-2) and 0.92 mu mol cm(-2), respectively. The hydrophilic pHEMA membrane had a swelling ratio of 58% (w/w) with a contact angle of 45.8 degrees. CB-immobilized and CB-immobilized-thionein-conjugated membranes were used in the Cd(II) removal studies. Cd(II) ion adsorption appeared to reach equilibrium within 30 min and to follow a typical Langmuir adsorption isotherm. The maximum capacity (q(m)) of the CB-immobilized membranes was 0.203 (mu mol Cd(II))cm(-2) membrane and increased to 1.48 (mu mol Cd(II))cm(-2) upon CB-thionein-complex conjugation. The pHEMA membranes retained their cadmium adsorption capacity even after 10 cycles of repeated use. (C) 2000 Society of Chemical Industry.eninfo:eu-repo/semantics/closedAccesspoly(2-hydroxyethyl methacrylate)affinity membraneCibacron Blue F3GApeptidethioneincomplex conjugationcadmiumhuman serumArticle49330230810.1002/(SICI)1097-0126(200003)49:3<3022-s2.0-0034156370Q1WOS:000085651300011Q2