Bayraktaroğlu, Alev GürolŞimşek, ÖzkanKürüm, AytülArıkan, ŞevketErgün, Emel2020-06-252020-06-252015Bayraktaroğlu, A. G., Şimşek, Ö., Kürüm, A., Arıkan, Ş., Ergün, E. (2015). Determination of alpha-naphthyl acetate esterase (ANAE) activity inperipheral blood leukocytes of pregnant, adult, and kitten Angora cats. Turkish Journal of Veterinary and Animal Sciences, 39(1), 57 - 61.1300-01281303-6181https://app.trdizin.gov.tr/publication/paper/detail/TWpFek1qVTNOdz09https://hdl.handle.net/20.500.12587/1427This study aimed to determine the alpha-naphthyl acetate esterase (ANAE) activity of peripheral blood leukocytes in pregnant, kitten, and adult Angora cats. In each group 9 healthy pregnant, adult, and kitten Angora cats constituted the material of the study. Optimum reaction was achieved after a 3 h incubation period at pH 5.8, by ANAE staining. Two types of reaction were observed in ANAE () T lymphocytes. The first reaction was a dot-like positivity pattern characterized by the presence of large granules, while the other was a granular positivity pattern characterized by the presence of 3 to 5 small granules. B lymphocytes reacted negatively in ANAE staining. The rate of ANAE () lymphocytes in pregnant, kitten, and adult Angora cats was 62.89 ± 1.29%, 68.37 ± 1.22%, and 77.71 ± 1.63%, respectively. In conclusion, the lowest rate of ANAE-positive lymphocytes was detected in the pregnant cats, depending on the maternal immune tolerance supported by hormonal mechanisms. An increased rate of ANAE-positive lymphocytes was detected in the kittens, and the highest rate was measured in the adults, in parallel with the development of the immune system.eninfo:eu-repo/semantics/openAccessZiraat MühendisliğiArticle3915761213257