Tastan, SametGunay, KubraArslan, MetinYigitoglu, MustafaGok, Zehra Gun2025-01-212025-01-2120230170-08391436-2449https://doi.org/10.1007/s00289-022-04613-4https://hdl.handle.net/20.500.12587/24595In this work, the glycidyl methacrylate-grafted (GMA) PET fibers were modified with hexamethylenediamine (HMDA) attachment and Cu2+ adsorption to synthesize an effective adsorbent for removal of bovine serum albumin (BSA) from the aqueous environment. The original PET fibers were grafted with GMA monomers (PET-g-GMA) at 30%, 50%, 100%, 150% and 200% grafting yields, and HMDA was covalently connected to the fibers through to the epoxy groups. The obtained fibers were characterized with the scanning electron microscopy (SEM), Fourier transform infrared (FTIR) and nuclear magnetic resonance spectroscopy (C-13-NMR) techniques. Afterward, Cu2+ ions were adsorbed to graft copolymers modified with HMDA and it was observed that the protein adsorption ability of Cu2+ ions containing fibers increased. In the adsorption study performed with PET-g-GMA and PET-g-GMA fibers modified with HMDA, the highest Q value was found to be 1.7 and 1.98, respectively, while this value was found to be 16.4 for HMDA-modified PET fibers containing Cu2+ ions. In the adsorption experiments, it was observed that the fibers with the highest BSA adsorption capacity were those modified (with HMDA and Cu2+) PET-g-GMA fibers that have 200% grafting yield. The effects of various parameters on BSA adsorption with the fibers with the highest adsorption capacity were investigated. In the experiment carried out with the selected fibers, pH optimization was made in the range of pH 3-9 and the optimum pH value was found to be pH 5. The Q value of the fiber at this pH was found to be 24.85 mg/g. During the adsorption process carried out at pH 5, it was found that the amount of adsorbed BSA reached equilibrium after the 9 h. Temperature optimization was carried out between 25 and 55 & DEG;C at optimum pH and incubation time. Q values were found as 24.8 mg/g at 25 & DEG;C, 44 mg/g at 35 & DEG;C, 47 mg/g at 45 & DEG;C and 22 mg/g at 55 & DEG;C. According to these results, the temperature at which the maximum adsorption took place was determined as 45 & DEG;C. In the next step where the initial protein concentration upon adsorption was investigated, it was concluded that the fibers reached saturation after an initial concentration of 200 ppm BSA concentration. As a result of the desorption study carried out for 2 h in acetate buffer containing 1 M NaCl at 25 & DEG;C, it was observed that approximately 85% of the loaded protein desorbed from the fibers after 75 min. It has been seen that the modified PET-g-GMA fibers used as adsorbent in this study can be used in separation and purification processes of BSA by adsorption process, due to its advantages such as high production, low cost, high processability, and easy modification.eninfo:eu-repo/semantics/closedAccessPoly(ethylene terephthalate) fibers; Bovine serum albumin; Hexamethylenediamine; Glycidyl methacrylateArticle809105691058910.1007/s00289-022-04613-42-s2.0-85143116673Q1WOS:000892917600003Q2