Somuncu, SalihÇakmak, MuratErdoğan, SibelCağlayan, OsmanAkman, HülyaKaya, Murat2020-06-252020-06-252006closedAccess0919-8172https://doi.org10.1111/j.1442-2042.2006.01355.xhttps://hdl.handle.net/20.500.12587/3795Objective: We aimed to detect the preventive effects of trapidil in ischemia reperfusion (IR) injury due to testicular torsion and detorsion. Methods: Forty prepubertal albino rats were used. In the IR group, torsion was created by rotating the left testis over 2 h, and detorsion was done by untwisting the testis. Bilateral orchiectomies were performed after 4 h. In study group, 2-h torsion was performed and trapidil was administered as a single dose I h before detorsion. Bilateral orchiectomies were performed after 4 h. In the sham group, a sham operation was done. In the sham plus trapidil group, a sham operation was done and trapidil was administered as a single dose. Testicular tissue malondialdehyde (MDA), nitric oxide (NO) and total sulfhydryl (T-SH) levels were determined for each group. The grades of interstitial injury were determined in histopathologic examination. Results: The NO and MDA levels in the IR group were significantly higher than the study, sham and sham plus trapidil groups in the left testis (P < 0.05 P < 0.001 and P < 0.001, respectively). A statistical difference was not found among Study, sham and sham plus trapidil groups in the left testis in NO and MDA levels (P > 0.05). The T-SH level in the study group was significantly higher than in the IR. sham and sham plus trapidil groups in left testis P < 0.05). In the IR group (left testis), grade I interstitial injury was 30% (3/10), grade 2 injury was 60% (6/10) and grade 3 injury was 10% (1/10). In the study group (left testis), grade I interstitial injury was 30% (3/10) and there was no injury in 70% (7/10). Conclusion: Trapidil decreased free oxygen radical formation in testicular torsion and detorsion, and attenuated histopathological damage in the ipsilateral twisted testis.eninfo:eu-repo/semantics/closedAccessfree oxygen radicalsischemia-reperfusion injurytestis torsion-detorsiontrapidilArticle13560160510.1111/j.1442-2042.2006.01355.x2-s2.0-3364678644416771732Q1WOS:000237600700020Q4