New pathway in rheumatic mitral valve disease: Cytochrome P450 and glutathione S transferase isozyme expression

Abstract

Objective: Cytochrome P450 (CYP)1A1, glutathione S-transferase pi (GSTP1) and omega (GSTO1) isozymes were evaluated and compared in patients with the diagnosis of rheumatic mitral valve disease and ischemic mitral valve insufficiency to find out the relationship of the oxidative stress with rheumatic mitral valve disease. Materials and methods: The control group consisted of patients operated on due to ischemic mitral valve insufficiency (group I, n:14) while study group consisted of the patients operated on with the diagnosis of rheumatic mitral valve disease (group II, n:29). Mitral valve materials were stained with hematoxylin and eosin. CYP1A1, GSTP1, and GSTO1 immunohistochemical markers were studied. Results: 20.7% of GSTP1 isozyme protein expression was seen in the study group; however, no expression was detected in the control group. This finding was statistically significant in terms of GSTP1 isozyme. No statistically significant differences in the level of GSTO1 and CYP1A1 protein expression between the study and control groups were observed. Conclusion: In this study, we found out that GSTP1 isozyme may be related to rheumatic mitral valve disease. A strategy that would help prevent oxidative stress in patients with rheumatic mitral valve disease can be a so valuable means to affect disease progression. © 2020 De Gruyter. All rights reserved.