dc.contributor.author | Temoçin Z. | |
dc.date.accessioned | 2021-01-14T18:16:12Z | |
dc.date.available | 2021-01-14T18:16:12Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 1567-5394 | |
dc.identifier.uri | https://doi.org/10.1016/j.bioelechem.2019.04.021 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12587/12998 | |
dc.description | PubMed: 31102940 | en_US |
dc.description.abstract | The aim of this study was to demonstrate the usability of ?-carrageenan in the accumulation of a layer by layer protein film on a gold electrode. It is known that in terms of bioelectronics there are significant advantages to functional biofilms which are formed as a result of the interaction between different layer biomolecules. In this study, ?-carrageenan (?-CG) was employed as the polyanion for the construction of the electroactive multilayer protein thin films. The protein layers were built up by successive adsorption of ?-CG and cytochrome c (cyt c) based on the opposite charge on a modified gold electrode with a cyt c monolayer. Electron transport between the cyt c layers was established without the hindrance of ?-CG. The highest electron transfer capability was achieved when the pH value of the solutions was 5, and the ?-CG concentration was a 0.25 mg mL?1. Whilst the amount of cyt c on the electrode surface was 10.87 pmol cm?2 in the monolayer structure, the amount was calculated to be 158.01 pmol cm?2 with the formation of 8-bilayer [?-CG/cyt c] structures over the cyt c monolayer. The multilayer protein film was also characterized using electrochemical impedance spectroscopy, ellipsometry, and UV–visible absorption spectroscopy. This novel biofilm allows a high amount of electron transfer and can be used as an immobilization platform for biosensor applications. © 2019 Elsevier B.V. | en_US |
dc.description.sponsorship | 2012-25 | en_US |
dc.description.sponsorship | This work was supported by the Research Foundation of Kırıkkale University ( 2012-25 ). | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Elsevier B.V. | en_US |
dc.relation.isversionof | 10.1016/j.bioelechem.2019.04.021 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Carrageenan | en_US |
dc.subject | Cytochrome c | en_US |
dc.subject | Electron transfer | en_US |
dc.subject | Electrostatic interaction | en_US |
dc.subject | Multilayer film | en_US |
dc.title | Fabrication of a ?-carrageenan-based electroactive cytochrome c multilayer thin film by an electrostatic layer-by-layer assembly | en_US |
dc.type | article | en_US |
dc.contributor.department | KKÜ | en_US |
dc.identifier.volume | 129 | en_US |
dc.identifier.startpage | 34 | en_US |
dc.identifier.endpage | 41 | en_US |
dc.relation.journal | Bioelectrochemistry | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |