Hiperprolaktineminin Total Oksidan, Total Antioksidan, Total Thiol ve Native Thiol Düzeyleri Üzerindeki Etkisinin İncelenmesi

Abstract

Aim: The aim of this study was to investigate the effects of hyperprolactinemia on total antioxidant levels (TAS), total oxidant levels (TOS), total thiol and native thiol levels. Background: Prolactin; it is a peptide hormone synthesized from the anterior pituitary by pituitary lactotrophs in both physiological and pathological conditions. Elevated prolactin (>30 ng/mL) is a common syndrome called "hyperprolactinemia". It affects both sexes. It is characterized by classical amenorrhea (in women) -- galactorrhea and hypogonadotropic hypogonadism (in both sexes) that cause infertility in both sexes. It is stated that prolactin is associated with functions of reducing cytosolic reactive oxygen species (ROS) and maintaining antioxidant capacity, apart from its known functions such as initiating milk secretion. ROS are produced by cells in normal metabolic and physiological processes. Oxygen is converted to water in mitochondria as a result of electron transport chain reactions. In this metabolic process, 2-3% of the oxygen in the mitochondria does not turn into water, but forms a source for the formation of radicals such as oxygen-derived superoxide, hydrogen peroxide and hydroxyl radical. ROS can cause harmful oxidative reactions in organisms that remove them by enzymatic and non-enzymatic antioxidative mechanisms. Under certain conditions, an increase in oxidants and a decrease in antioxidants cannot be prevented, and the oxidative/antioxidative balance shifts towards the oxidative state. As a result, oxidative stress develops, which plays a role in the formation of a large number of diseases. Although it is possible to measure the plasma concentrations of oxidant molecules separately, this is not feasible as these molecules can affect each other. Therefore, the total oxidant status (TOS) measurement, which reflects the overall oxidant status, has been developed. TAS measurement is similarly more informative than individual measurements of antioxidants. One of the important antioxidant molecules in the cell is thiol (thiol) groups. Intracellular thiols, including glutathione and thioredoxin, present in millimolar concentrations within cells, are essential for maintaining the highly reduced intracellular environment. Thiols also form an important component in the antioxidant defense of the cell. Thiol/disulfide homeostasis (TDH) is a marker of oxidative stress. Materials and Methods: This study will consist of approximately 30 hyperprolactinemia patients and 30 healthy volunteers who applied to the Kırıkkale University Medical Faculty Hospital Obstetrics and Gynecology clinic. Necessary diagnoses will be made by Dr. Nevin Sağsöz. In order to carry out the study, blood samples will be taken from all volunteers and their age, weight, height, etc. demographic characteristics such as galactorrhea and amenorrhea, menstrual patterns will be recorded. The correlation between the data obtained from the study and prolactin levels will also be examined. TAS and TOS analyzes will be made using the serum obtained from blood samples from volunteer individuals. TAS, TOS, native thiol and total thiol analyzes will be determined using the calorimetric measurement kits.