Changes in neuropeptide y tissue concentration in the wall of the rat urinary bladder after acute distension

Abstract

Neuropeptide Y (NPY) is known to be associated with the adrenergic system. The relationship among the late micturition disorders following acute urinary distension, the adrenergic system and NPY was investigated. A total of 90 rats were included in the study of which 30 acted as the control group. Acute urinary distension was created in 60 rats. The NPY concentration within their bladders was assessed by the use of radioimmunoassay (RIA) at 3 h after distension and subsequently on days 2, 7 and 21, then the third and sixth months. The NPY concentrations assessed in the third and sixth months were compared with the control group in the same age group. By means of the RIA method, a substantial decline of NPY concentration was observed at 2 days after distension, while the concentration started to increase after day 7 (P=0.003). This increase continued until the twenty first day (P=0.004). However, a significant decline was maintained when compared to the concentration before distension. In the third and sixth months, a significant decline were observed in the NPY concentration in comparison to the control group (P=0.004 and P=0.005, respectively). Early and late micturition disorders experienced after acute urinary distension may be the result of adrenergic denervation which may be related to NPY.