The contribution of thiamine, pyridoxine and cyanocobalamine combination on nerve regeneration in rats with experimentally induced sciatic injury

Abstract

The aim of this study was to investigate the role of thiamine, pyridoxine and cyanocobalamine combination on nerve regeneration after experimentally induced crush injury in thirty adult, male wistar rats. Sciatic function index and Toe spreading reflex and nerve conduction studies on exposed sciatic nerve were carried out before sciatic crush injury and after three weeks of treatment. The experiment group received thiamine (33 mg kg(-1)), pyridoxine (33 mg kg(-1)) and cyanocobalamine (0.5 mg kg(-1)) injections intramuscularly for 3 weeks and the control group received equal volumes of saline injections. Toe-spreading reflex were better in the experiment group than the controls after treatment (p<0.05). There was no significant difference in regard to DL, DCMAP, PCMAP and NCV between both groups (p<0.05). In conclusion the beneficial effect of thiamine, pyridoxine and cyanocobalamine combination was not found in regard to the electrophysiological parameters measured. However clinical improvement was superior in the experiment group than the control group.