dc.contributor.author | Öktem, Hüseyin Avni | |
dc.contributor.author | Bayramoğlu, Gülay | |
dc.contributor.author | Özalp, V. Cengiz | |
dc.contributor.author | Arica, M. Yakup | |
dc.date.accessioned | 2020-06-25T17:44:02Z | |
dc.date.available | 2020-06-25T17:44:02Z | |
dc.date.issued | 2007 | |
dc.identifier.citation | closedAccess | en_US |
dc.identifier.issn | 8756-7938 | |
dc.identifier.issn | 1520-6033 | |
dc.identifier.uri | https://doi.org10.1021/bp0602505 | |
dc.identifier.uri | https://hdl.handle.net/20.500.12587/3989 | |
dc.description | Ozalp, Veli Cengiz/0000-0002-7659-5990 | en_US |
dc.description | WOS: 000243927600021 | en_US |
dc.description | PubMed: 17269682 | en_US |
dc.description.abstract | A DNA aptamer specific for Thermus aquaticus DNA polymerase (Taq-polymerase) was immobilized on magnetic beads, which were prepared in the presented study. The effect of various parameters including pH, temperaturem and aptamer concentration on the immobilization of 5'-thiol labeled DNA-aptamer onto glutaric dialdhyde activated magnetic beads was evaluated. The binding conditions of Taq-polymerase on the aptamer immobilized magnetic beads were studied using commercial Taq-polymerase to characterize the surface complexation reaction. Efficiency of affinity magnetic beads in the purification of recombinant Taq-polymerase from crude extracts was also evaluated. For this case, the enzyme "recombinant Taq-DNA polymerase" was cloned and expressed using an Amersham E. coli GST-Gene Fusion Expression system. Crude extracts were in contact with affinity magnetic beads for 30 min and were collected by magnetic field application. The purity of the eluted Tag-polymerase from the affinity beads, as determined by HPLC, was 93% with a recovery of 89% in a one-step purification protocol. Apparently, the system was found highly effective as one step for the low-cost purification of Taq-polymerase in bacterial crude extract. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Wiley | en_US |
dc.relation.isversionof | 10.1021/bp0602505 | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.title | Single-step purification of recombinant Thermus aquaticus DNA polymerase using DNA-aptamer immobilized novel affinity magnetic beads | en_US |
dc.type | article | en_US |
dc.identifier.volume | 23 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.startpage | 146 | en_US |
dc.identifier.endpage | 154 | en_US |
dc.relation.journal | Biotechnology Progress | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |