Evaluation Of Oxidative Stress Markers And Antioxidant Status In The Alzheimer Disease, Vascular Dementia And The Parkinson Disease

Abstract

Oxidative stress may be involved in pathogenesis of neurodegenerative diseases. We aimed to measure MDA, NO, 8-OHdG and TAC, SOD and CAT levels in the Alzheimer disease, Parkinson disease and vascular dementia, and evaluate oxidant and antioxidant status in these diseases. Oxidative stress parameters were measured in 20 patients with vascular dementia, 20 patients with the Alzheimer disease, 20 patients with the Parkinson disease and 20 matching controls. MDA, NO and TAC were assayed with spectrophotometric methods. 8-OHdG was quantitated by the Elisa method. SOD and CAT were assayed with colorimetric methods. MDA, NO, 8-OHdG and SOD levels in total patient group were higher (p = 0.039, 0.041, 0.021 and 0.045, respectively), while TAC levels were lower (p = 0.010) as compared with controls. When compared to controls, the following were higher: MDA in the Parkinson disease (p = 0.036); NO in the Alzheimer disease (p = 0.028). However, TAC in the Alzheimer disease (p = 0.024) were lower. We observed negative correlations between TAC and MDA in the Parkinson disease (r = -0.496, p = 0.026); between SOD and 8-OHdG in the Alzheimer disease (r = 0.526, p = 0.017). These findings support the idea that the oxidative stress may play an important role in the pathogenesis of neurodegenerative diseases. This preliminary study shows an increase in free radical production in the Alzheimer disease and Parkinson disease. Also, there is a defect in the antioxidant defense system, which may lead to oxidative damage in patients with the Alzheimer disease.