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dc.contributor.authorSoltanzadeh, Hossein
dc.contributor.authorAcik, Leyla
dc.contributor.authorTurk, Mustafa
dc.contributor.authorHoushmand, Massoud
dc.contributor.authorShahsavari, Gholamreza
dc.date.accessioned2020-06-25T18:30:01Z
dc.date.available2020-06-25T18:30:01Z
dc.date.issued2018
dc.identifier.citationSoltanzadeh H, Açık L, Türk M, Houshmand M, Shahsavari G (2018). Antimicrobial, antioxidant, cytotoxic and apoptotic activities of Satureja khuzestanica. Gazi Medical Journal, 29(3), 264 - 270.en_US
dc.identifier.issn2147-2092
dc.identifier.urihttps://doi.org/10.12996/gmj.2018.75
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7532
dc.descriptionWOS: 000437529600029en_US
dc.description.abstractObjective: Natural products with antimicrobial effect are currently investigated in order to eliminate the use of synthetic antibiotics that cause the resistance of microorganisms. This study determines the antibacterial, antioxidant activity, and also the cytotoxic and apoptotic effect of Satureja khuzestanica on various cancer cell lines. Materials and methods: The individual constituents were identified by their identical retention indices referring to known compounds from the literature. Methanol and ethanol extracts of S. khuzestanica (25 and 50 mg/(mL)) were screened against four Gram-positive and four Gram-negative bacteria. The plant extracts were tested for their antioxidant activity against 2,2-diphenyl1- picrylhydrazyl (DPPH) radical. The interaction between the extracts and plasmid DNA were analyzed by agarose gel electrophoresis. In addition, the cytotoxic activity of the extracts was evaluated on MCF-7, DLD-1, osteosarcoma, and fibroblast cell line. Finally, gene expression of caspase-3, Bax and Bcl-2 were investigated by real-time PCR. Results: Our result indicated that both extracts showed good inhibitory activity against both Gram-negative (Escherichia coli ATCC 35218) and positive (Bacillus subtilis ATCC 6633) bacteria. In addition, the methanol extract of S. khuzestanica had strong antioxidant activity (IC50= 37.0 +/- 0.3 mu g/mL). The extracts showed a strong effect on plasmid DNA. The methanol extract of S. khuzestanica showed a good concentration-dependent cytotoxicity. Finally, IC50= 47.00a +/- 1.21 mu g/mL, ethanol, and water extract had apoptotic effect in MCF-7 cell line. Discussion: MCF-7 was detected as the most sensitive cell line therefore further studies should be done on this plant extract as a potential anticancer agent against breast cancer.en_US
dc.description.sponsorshipGazi UniversityGazi Universityen_US
dc.description.sponsorshipThis study was supported in a PhD program by Gazi Universityen_US
dc.language.isoengen_US
dc.publisherGazi Univ, Fac Meden_US
dc.relation.isversionof10.12996/gmj.2018.75en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSatureja khuzestanicaen_US
dc.subjectplasmid DNAen_US
dc.subjectMCF-7en_US
dc.subjectcytotoxicityen_US
dc.titleAntimicrobial, antioxidant, cytotoxic and apoptotic activities of Satureja khuzestanicaen_US
dc.typereviewen_US
dc.contributor.departmentKırıkkale Üniversitesien_US
dc.identifier.volume29en_US
dc.identifier.issue3en_US
dc.identifier.startpage264en_US
dc.identifier.endpage270en_US
dc.relation.journalGazi Medical Journalen_US
dc.relation.publicationcategoryDiğeren_US


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