Anti Wnt-1 Monoclonal Antibody's Conjugated with Gold Nanoparticles, Induced Apoptosis on MCF-7 Breast Cancer Cell Lines

Abstract

Wnt-beta Catenin Pathway has an important role in many cancers. Wnt-1 protein from Wnt protein family that regulate this pathway has a special effect on the development of breast cancer. Monoclonal antibodies attach to metal nanoparticles have an important role in diagnosis and treatment of cancers. In this study, Anti Wnt-1 monoclonal antibody was conjugated to the gold nanoparticles synthesized by Turkevich method. Conjugation was achieved using EDC-NHS method. The density of the monoclonal antibodies that bonded to gold nanoparticles was measured by Roche Cobas Integra 400 Plus device. MCF-7 breast cancer cell line was treated with conjugated nanoparticles for 48 h then Double Staining method was used to detect apoptosis cells. The results showed that inhibition of Wnt-1 protein in extracellular matrix causes apoptosis and gold nanoparticles have a positive effect on Anti Wnt-1 monoclonal antibodies and this positive effect causes apoptosis to increase rate in conjugating nanoparticles.