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    Biochemical and Molecular Characterization of Strontium-resistant Environmental Isolates of Pseudomonas fluorescens and Sphingomonas paucimobilis
    (Taylor & Francis Inc, 2013) Özer, Gamze; Ergene, Aysun; İçgen, Bülent
    Strontium resistance was investigated in strains isolated from the river Kzlrmak which has been affected by industrial and agricultural heavy metal pollution from the surrounding facilities and domestic effluents from the city Krkkale, Turkey. Strontium-resistant isolates with a minimal inhibitory concentration of 2000mg L-1 were isolated and identified as Pseudomonas fluorescens and Sphingomonas paucimobilis. Both isolates were shown to be resistant to other heavy metals like aluminum, lithium, barium, nickel and resistance to the antibiotics like aztreonam and pefloxacin. Plasmid DNA analysis, transformation and curing results revealed that the strontium resistance ability of both isolates was chromosome-encoded. Outer membrane and total protein isolation results descriptively showed that not only membrane porins but also other proteins were effective in the strontium resistance in Pseudomonas fluorescens and Sphingomonas paucimobilis.
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    The cytotoxic effect of endemic Centaurea fenzlii Reichardt on colon cancer cell lines
    (Georg Thieme Verlag Kg, 2012) Yırtıcı, Ümit; Yilmaz, F.; Serim, Gamze; Kirimer, Neşe; Ulukaya, Engin; İçgen, Bülent; Ergene, Aysun
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    Öğe
    Endemik centaurea fenzlii reichardt türünün kolon ve meme kanseri hücre dizileri üzerindeki sitotoksik ve apoptotik etkisi
    (2013) Ergene, Aysun; Kırımer, Neşe; İçgen, Bülent; Yırtıcı, Ümit
    [Abstract Not Available]
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    Kırıkkale-Kızılırmak'dan civa dirençli bakterilerin izole edilmesi ve tanımlanması
    (2012) İçgen, Bülent
    [Abstract Not Available]
  • Küçük Resim
    Öğe
    Pretreatment of poultry litter improves Bacillus thuringiensis-based biopesticides production
    (Elsevier Sci Ltd, 2010) Özcan, Orhan; İçgen, Bülent; Özcengiz, Gülay
    Pretreated poultry litter was used in batch cultures for the production of Bacillus thuringiensis (Bt)-based biopesticide of lepidoptera- and diptera-specific Cry1 and Cry2, diptera-specific Cry4Ba and Cry11Aa and coleoptera-specific Cry3Aa toxins by Bt subsp. kurstaki 81, subsp. israelensis HD500 and subsp. tenebrionis 3203, respectively. Bt kurstaki 81 showed improved growth and produced more toxin in this medium as compared to other subspecies. Base and acid hydrolysis were tested as the methods of substrate Pretreatment. The use of poultry litter pretreated with 2 N HCl yielded 94% more bioinsecticidal protein than 2 N NaOH-pretreated poultry litter when Bt kurstaki 81 was cultured. With appropriate pretreatment, poultry litter demonstrated potential as a valuable raw material for a low-cost complex medium to produce Bt-based biopesticides. (C) 2009 Elsevier Ltd. All rights reserved.