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  • Küçük Resim
    Öğe
    Effect of Epidermal growth factor on ın vitro maturation of cat oocytes recovered from ovaries at follicular and luteal stages
    (2009) Polat, Bülent; Acar, Duygu Baki; Macun, Hasan Ceyhun; Korkmaz, Ömer; Çolak, Armağan; Baştan, Ayhan; Akçay, Aytaç
    Bu çalışmada, seksüel siklusun folliküler ve luteal fazında bulunan kedi ovaryumlarından toplanan oositlerin nükleer maturasyonu üzerinde IVM vasatına çeşitli konsantrasyonlarda katılan EGF’nin etkilerinin belirlenmesi amaçlandı. Çalışmada folliküler ve luteal dönemde bulunan toplam 42 kediden alınan 612 kumulus oosit kompleks kullanıldı. Oositler, (1) TCM 199 (EGF 0); (2) TCM 199 10 ng/ml EGF; (3) TCM 199 50 ng/ml EGF olarak 3 gruba ayrılıp IVM vasatına alındı ve %5 CO2’li ortamda 48 saat süreyle mature edildi. Kromozomal analizlere göre her bir oosit germinal vezikül (GV), Metafaz I-II (M I-II) ve dejenere olarak adlandırıldı. EGF 50 grubunda, luteal dönemdeki ovaryumlardan alınan oositlerin yüksek oranda (%41.7) GV aşamasında kaldığı gözlendi (P0.05). EGF 10 grubunda folliküler dönemdeki oositlerin önemli oranda (%37.9) M II aşamasına ulaştığı saptandı (P0.05). Tüm EGF gruplarında luteal dönemdeki ovaryumlardan alınan oositlerin folliküler döneme göre yüksek oranda M II aşamasına ulaştığı görüldü (P0.05). Sonuç olarak sunulan çalışmada IVM vasatına katılan düşük dozda EGF’nin (10 ng/ml) EGF katılmayan gruba kıyasla maturasyonu olumlu yönde etkilediği gözlendi. Ancak maturasyon vasatına yüksek miktarda (50 ng/ml) katıldığında ise kedi oositlerinin IVM’si üzerinde olumsuz etkilerinin olduğu ve luteal dönemdeki ovaryumlardan toplanan oositlerin folliküler döneme göre IVM için daha uygun olduğu kanısına varıldı.
  • Küçük Resim
    Öğe
    Ultrasonographic Evaluation of Mammary Tumors in Bitches
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2009) Bastan, Ayhan; Ozenc, Erhan; Yagci, Ilknur Pir; Acar, Duygu Baki
    The aim of this study was to characterize the ultrasonographic patterns of benign and malignant mammary tumors and to evaluate the efficacy of ultrasonographic imaging to distinguish benign and malignant tumors in dogs. Shape, margins, width-to-craniocaudal (CC) dimension ratio, echotexture, echogenicity, presence of calcification, presence of pseudocapsule, size (the deepest and longest axis) and acoustic transmission Of tumors were evaluated. The ultrasonography features and the tissue diagnosis of benign tumor were oval or round (80% of masses with this feature were benign), and circumscribed margins (95% were benign). The features most predictive of a malignant tumor diagnosis were spiculated or microlobulated margins (76% of masses with this feature were malignant), and irregular shape (78% were malignant). No differences between the benign and malignant groups for width-to-CC dimension ratio >1.4 were observed. The benign tumors were seen in different distribution of beign hypoechoic (30%), isoechoic (35%) or varied in echogenicity. It was concluded that, the ultrasonography could be an extremely useful tool for the preevaluation of canine mammary gland tumors, especially in relation to the evaluation of tumor size, shape, border irregularity, echotexture, internal echogenicity and acoustic transmission.
  • Küçük Resim
    Öğe
    Use of early conception factor test for determining pregnancy and embryonic mortality status of dairy cows
    (2007) Baştan, Ayhan; Özenç, Erhan; Macun, Hasan Ceyhun; Acar, Duygu Baki; Güngör, Örsan
    The aim of this study was to evaluate the accuracy of the ECF test for detecting the pregnancy status and embryonic mortality and to compare the reliability of ECF test from among ultrasonography and serum progesterone level. In this study, two groups were designed: the study group (n = 15) and control group (n = 9). All cows were observed for estrus activity four times daily. Cows in the study group were inseminated. After insemination, at the 7, 20, 30 and 45th days ECF test and ultrasonographic examination were applied to check the pregnancy status. Cows in the control group were not inseminated and examination procedure was performed like in the study group. Twenty days after insemination, pregnant positive cows that had been determined by ultrasonography were designated the study group. Twenty days after insemination, ECF test were applied and progesterone levels were determined in the serum samples obtained from pregnant positive cows. Fifteen cows in the study group were checked 20 days after insemination and determined pregnant. Their pregnancy status was confirmed 20 days after insemination by using ultrasonography. In the 30 th and 45th days ultrasonography was repeated, after which 13 cows were determined pregnant. In the serum of these two cows progesterone levels fell under 2 ng/ml. However, in the 20th day these cows' progesterone levels was higher than 2 ng/ml, in two cows embryonic death occurred. In cows which were determined as pregnant by ultrasonography at the 20th day, the ECF test was applied at the 7th day and 10 cows from this group had a positive reaction (66.7%). Test specificity, PPV and NPV results were 44.4%, 66.7% and 44.4% respectively; at the 20th day the ECF test was positive for 9 cows (60%), specificity, PPV and NPV results were 33.3%, 60.0% and 33.3%; at the 30th day, the ECF test was positive for 12 cows (92.3%), test specificity, PPV and NPV results were 45.5%, 66.7% and 83.3%; at the 45th day, 10 cows (76.9%), test specificity, PPV and NPV results were 54.5%, 66.7% and 66.7% respectively. Between the study groups, the ECF test accuracy at the 7th and 20th days were found lower than at the other days. The test's accuracy was determined the highest at the 30th day (70.8%), and the lowest at the 20th day (50%). The results show that ECF test is an unreliable method for pregnancy diagnosis and for determining embryonic death in dairy cows and these data indicate that the current ECF test cannot accurately identify the nonpregnant cows.