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    Öğe
    Association Between TP53 Gene Polymorphism and Obesity
    (Society of Pharmaceutical Sciences of Ankara (FABAD), 2021) Ci?Han, Mehmethan; Buluş, Hakan; Di?Ri?Can, Onur; Oğuztüzün, Serpil; Öztürk, Doğan; Ünsal, Abdulkadir; Ada, Ahmet Oğuz
    Obesity is a chronic disorder with increasing prevalence worldwide and occurs when energy intake is greater than energy expenditure. Obesity is one of the factors that cause oxidative stress and arises from an imbalance between the reactive oxygen species (ROS) and the cell's antioxidant defense system. Increasing ROS in obesity, influencing the hypothalamic neurons, affects hunger and satiety control, so correspondingly on body weight control. When ROS amount increases, through DNA, protein and lipid oxidation, cell damage, necrosis, and apoptosis take place. Tumor protein p53, the guardian of the genome, is responsible for the regulation of genes involved in apoptosis as well as energy generating metabolic pathways. In our study, we investigated the TP53 (Arg72Pro) polymorphism in 151 patients diagnosed with obesity. TP53 mutation (rs1042522) was determined by real-time PCR. In 8 patients, the TP53 mutation was identified as carrying heterozygous (Arg72Pro) and in 143 patients carrying homozygous (wild type) (Arg72Arg). No individual with a homozygous mutant (Pro72Pro) genotype was found in the studied group. Associations between TP53 genotypes and clinical obesity parameters such as body mass index, thyroid stimulating hormon, glucose, postprandial blood sugar, triglyceride and cholesterol levels were compared statistically. According to the results of statistical analysis, it was observed that TP53 polymorphism was associated with insulin level. Genotype frequencies were also compared with previous studies performed in control populations and found to be different. This study shows that there may be a relationship between TP53(Arg72Pro) polymorphism and obesity. © 2021 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.
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    Öğe
    Investigation of GSTM1 and GSTT1 polymorphisms in obesity patients under bariatric surgery
    (Society of Pharmaceutical Sciences of Ankara (FABAD), 2021) Ünsal, Abdulkadir; Buluş, Hakan; Dirican, Onur; Oğuztüzün, Serpil; Öztürk, Doğan; Cihan, Mehmethan; Ada, Ahmet Oğuz
    Obesity is a chronic disorder with increasing prevalence worldwide and occurs when energy intake is more than energy expenditure. Obesity is one of the factors that cause oxidative stress and arises from an imbalance between the reactive oxygen species ROS and the cell’s antioxidant defense system. Increasing ROS in obesity, influencing the hypothalamic neurons, affect hunger and satiety control, so correspondingly on body weight control. When ROS amount increases, through DNA, protein, and lipid oxidation, cell damage, necrosis, and apoptosis take place. Oxidative stress increment in adipose tissue causes the development of metabolic syndrome in obese people. Also, weight loss due to calorie restriction or exercise reduces oxidative stress. Mitochondria is the essential source for ROS formation. In the electron transfer system, reactive oxygen species forming due to oxidative phosphorylation reactions are involved in various physiological processes such as cell proliferation and differentiation. Glutathione S-transferase M1 and T1 genes encode enzymes that have oxidant-scavenging activities. Deletion polymorphisms in these genes cause the absence of their corresponding enzymes. In this study, we investigated the parameters associated with obesity such as body mass index (BMI), TSH, glucose, satiety blood glucose, triglyceride, and cholesterol levels, and deletion polymorphisms of GSTM1 and GSTT1 genes in 152 patients diagnosed with obesity in a Turkish population. No statistically significant relationship was found between the parameters studied in obese patients and GSTM1 and GSTT1 polymorphisms. More studies are needed to elucidate the relationship of GSTM1 and GSTT1 polymorphisms with obesity. © 2021 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.
  • [ X ]
    Öğe
    Küçük Hücreli Dışı Akciğer Kanserinde Glutatyon S-Transferaz M1 ve T1 Polimorfizmleri ve Protein İfadeler
    (2017) Kılıç, Murat; Ada, Ahmet Oğuz; Oğuztüzün, Serpil; Demirağ, Funda; Çelik, Sezgin; Bıçakcıoğlu, Pınar; İşcan, Mümtaz
    Amaç: Glutatyon S-transferaz (GST) GSTM1 ve GSTT1 genlerinde delesyon polimorfizmleri, karsinojenlerin detoksifikasyonunu azaltan ilgili enzimlerin yokluğuna neden olur. Aynı hastalarda polimorfizm ve protein ifadelerinin değerlendirildiği çalışmalar sınırlıdır. Bu nedenle, bu çalışmada, küçük hücreli dışı akciğer kanser (KHDAK) hastalarının akciğer dokularında, GSTM1 ve GSTT1 polimorfizmleri ile protein ifadeleri arasındaki ilişkinin incelenmesini amaçladık.Gereç ve Yöntemler: Protein ekspresyon ve gen delesyon çalışmaları için, 33 KHDAK'li hastanın arşiv dokularından elde edilen tümörlü ve çevresindeki normal doku çiftleri kullanıldı. Parafine gömülü dokularda, protein ekspresyonlarını belirlemek için immünohistokimyasal metod, gen delesyonlarını belirlemek için multipleks polimeraz zincir reaksiyonları yöntemi kullanıldı. Bulgular: GSTM1 ve GSTT1 gen delesyonlarına sahip hastalarda GSTM1 ve GSTT1 protein ifadeleri bulunmazken, GSTM1 ve GSTT1 genlerini taşıyan tüm hastaların akciğer dokularında protein ifadeleri saptanmıştır. GSTT1'in protein ifadesi, GSTM1 geninden yoksun olan hastaların tümünde, GSTM1 genine sahip olanlardan 2.0 kat daha yüksek olduğu gözlenmiştir (p=0.018). GSTM1'in protein ifadesi, GSTM1 genine sahip hastaların tümör dokularında, normal dokularından istatistiksel olarak daha yüksek belirlenmiştir (p=0.001). Sonuç: Bu sonuçlar, a) KHDAK'li hastaların GSTM1 ve GSTT1 protein ifadeleri ile gen delesyonları arasında bir ilişkinin olduğunu, b) GSTM1 geninin yokluğunda, tümör dokularında GSTT1 protein ifadesinin artışı bu dokularda GSTT1'in toksik elektrofilikleri detoksifiye etme kapasitesini arttırma eğiliminde olduğunu ve c) KHDAK'li hastaların normal dokularına kıyasla tümörlü dokularında GSTM1 protein ifadesinin daha yüksek olduğunu göstermektedir.