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    A comprehensive study of Helicobacter pylori infection: molecular analysis, antibacterial susceptibility, and histopathological examination
    (Springer, 2023) Büyük, Fatih; Karakaya, Emre; Akar, Mustafa; Kayman, Tuba; Tarhane, Serdal; Özcan, Hacer Ece; Çelebi, Özgür
    Helicobacter pylori is a pathogen associated with gastroduodenal diseases. This study aimed; (i) to investigate H. pylori presence by invasive tests in adult dyspeptic patients, (ii) to determine antibiotic susceptibility and genotypic characteristics of the H. pylori isolates, and (iii) to investigate the relationship between the H. pylori genotypes and the histopathological findings. In this cross-sectional study, gastric biopsy samples from 208 adult dyspeptic patients were used for culture, tissue Polymerase Chain Reaction (PCR), and histopathological analysis. Antibiotic susceptibility of the H. pylori isolates was analyzed by gradient method. Analysis of the virulence genes was performed by monoplex PCR. Genetic profiles (from A to H) were created based on the virulence genes presence. Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) was used for the genotyping of the H. pylori isolates. The mean age of the patients was 46 (& PLUSMN; 15) years and 128 (61.5%) of them were female. H. pylori positivity was detected by culture, tissue PCR and histopathological examination in 59 (28.4%), 114 (54.8%) and 81 (38.9%) patients, respectively. The overall prevalence of H. pylori was found to be 63% (131/208). All H. pylori isolates were susceptible to tetracycline and amoxicillin. The resistance rates for metronidazole, clarithromycin, levofloxacin, and rifampicin were 67.2%, 27.9%, 34.4% and 13.11%, respectively. Multi drug resistance (MDR) was detected at the rate of 45.9% (28/61). While the most common virulence gene was cagA (93.44%), the least common was vacAm1 (23%). The predominant genetic profile was profile A (47.5%). ERIC-PCR results revealed a total of 26 different patterns. A high prevalence of H. pylori was detected in adult dyspeptic patients as in developing countries. It was observed significant genotypic heterogeneity and virulence gene diversity within the isolates. A considerable resistance rate detected against antibiotics such as clarithromycin, metronidazole, and levofloxacin, which are frequently used in the eradication of H. pylori, should be taken into consideration when creating regional empirical treatment regimens.
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    A comprehensive study of Helicobacter pylori infection: molecular analysis, antibacterial susceptibility, and histopathological examination (vol 116, pg 1261, 2023)
    (Springer, 2023) Büyük, Fatih; Karakaya, Emre; Akar, Mustafa; Kayman, Tuba; Tarhane, Serdal; Özcan, Hacer Ece; Çelebi, Özgür
    [Abstract No tAvailable]
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    Correction: A comprehensive study of Helicobacter pylori infection: molecular analysis, antibacterial susceptibility, and histopathological examination (Antonie van Leeuwenhoek, (2023), 116, 12, (1261-1273), 10.1007/s10482-023-01868-3)
    (Springer Science and Business Media Deutschland GmbH, 2023) Buyuk, Fatih; Karakaya, Emre; Akar, Mustafa; Kayman, Tuba; Tarhane, Serdal; Ozcan, Hacer Ece; Celebi, Ozgur
    In the original publication of the article, the Fig. 1 text citation was incorrectly provided in the section “Virulence genes analysis”. However, it should have been Fig. 2. The Fig. 2 text citation was incorrectly provided in the section “Clinical data and diagnostic tests results”. However, it should have been Fig. 1. In the section “Antimicrobial testing results”, Fig. 2 text citation is removed. The figures 1 and 2 have been swapped correctly. The original article has been corrected. © 2023, Springer Nature Switzerland AG.
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    The canonical Brucella species-host dependency is changing, however, the antibiotic susceptibility profiles remain unchanged
    (Academic Press Ltd- Elsevier Science Ltd, 2023) Çelik, Elif; Kayman, Tuba; Büyük, Fatih; Sağlam, Aliye Gülmez; Abay, Secil; Akar, Mustafa; Karakaya, Emre
    Brucellosis is a chronic disease caused by Brucella species with a wide range of hosts, from marine mammals to terrestrial species, but with strict host preferences. With the zoonotic character, the prevalence of human brucellosis cases is a reflection of animal infections. This study aimed to identify 192 Brucella isolates obtained from various sources by Bruce-ladder PCR and to determine their antibiotic susceptibilities by gradient diffusion method (E-test). As a result of the PCR, all human isolates (n = 57) were identified as B. melitensis. While 58 (82.9%) of the cattle isolates were identified as B. abortus, 59 (90.8%) of the sheep isolates were identified as B. melitensis. In addition, 12 (17.1%) of the cattle isolates and 6 (9.2%) of the sheep isolates were determined as B. melitensis and B. abortus, respectively. The primary host change behavior of B. melitensis was 1.9 times higher than that of B. abortus. While gentamicin and ciprofloxacin susceptibilities of Brucella isolates were 100%, tetracycline, doxycycline, streptomycin, trimethoprim/sulfamethoxazole and rifampicin susceptibilities were 99%, 99%, 97.4%, 91.7% and 83.9%, respectively. The lowest sensitivity of the isolates was determined against to cefoperazone as 26%. A triple-drug resistance was detected in 1 B. abortus isolate that included simultaneous resistance to cefoperazone, rifampicin, and trimethoprim/sulfamethoxazole. The high susceptibility profiles we found against to antibiotics such as tetracycline, doxycycline gentamicin and ciprofloxacin, used widely in treatment, are encouraging. However, the change in the canonical Brucella species-primary host preference suggests the need to reconsider eradication program, including updating vaccine formulations.